Key features and details
- Rabbit polyclonal to Prickle 2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Prickle 2 antibody
See all Prickle 2 primary antibodies
DescriptionRabbit polyclonal to Prickle 2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human Prickle 2 aa 700-800 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: Y79; WERI.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).|
Tissue specificityExpressed in brain, eye and testis. Additionally in fetal brain, adult cartilage, pancreatic islet, gastric cancer and uterus tumors.
Involvement in diseaseEpilepsy, progressive myoclonic 5
Sequence similaritiesBelongs to the prickle / espinas / testin family.
Contains 3 LIM zinc-binding domains.
Contains 1 PET domain.
Cellular localizationNucleus membrane.
- Information by UniProt
- EPM5 antibody
- PRIC2 antibody
- PRIC2_HUMAN antibody
All lanes : Anti-Prickle 2 antibody (ab65964) at 1 µg/ml
Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 96 kDa
Observed band size: 96 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
ab65964 has not yet been referenced specifically in any publications.