Overview

  • Product name

  • Description

    Rabbit polyclonal to Prickle 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Prickle 2 aa 700-800 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90635)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: Y79; WERI.

Properties

Applications

Our Abpromise guarantee covers the use of ab65964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).

Target

  • Tissue specificity

    Expressed in brain, eye and testis. Additionally in fetal brain, adult cartilage, pancreatic islet, gastric cancer and uterus tumors.
  • Involvement in disease

    Epilepsy, progressive myoclonic 5
  • Sequence similarities

    Belongs to the prickle / espinas / testin family.
    Contains 3 LIM zinc-binding domains.
    Contains 1 PET domain.
  • Cellular localization

    Nucleus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EPM5 antibody
    • PRIC2 antibody
    • PRIC2_HUMAN antibody
    • Prickle homolog 2 (Drosophila) antibody
    • Prickle planar cell polarity protein 2 antibody
    • prickle-like 2 (Drosophila) antibody
    • prickle-like 2 antibody
    • Prickle-like protein 2 antibody
    • PRICKLE2 antibody
    see all

Images

  • All lanes : Anti-Prickle 2 antibody (ab65964) at 1 µg/ml

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 96 kDa
    Observed band size: 96 kDa
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes

References

ab65964 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Sample
Mouse Tissue sections (E9.5 mouse embryo section)
Specification
E9.5 mouse embryo section
Permeabilization
Yes - PBST (PBS/0.1% v/v TritonX100 (sigma))
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 30 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up