Key features and details
- Rabbit polyclonal to PRIM2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRIM2 antibody
See all PRIM2 primary antibodies
DescriptionRabbit polyclonal to PRIM2
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- ICC/IF: HepG2 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab220252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
RelevancePRIM2 synthesizes small ribonucleotide primers for the initiation of DNA replication. The eukaryotic primase is a heterodimer of a small and a large subunit, p49 and p58 respectively. These two subunits purify as a complex tightly bound to DNA polymerase alpha. This tight association implicates polymerase alpha as the lagging-strand DNA polymerase in replication. Uniquely, primases are able to synthesize nucleotides de novo on a template by the formation of an initial dinucleotide. PRIM2 initiates synthesis with a triphosphate purine moiety at the 5'-end. After synthesis of 7-10 ribonucleotides, the primer template is translocated intramolecularly to the active site of the DNA polymerase alpha subunit. The p49 subunit of PRIM2 contains the catalytic active site. The human primase subunits are 90% identical in amino acid sequence to the mouse homologs.
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ab220252 has not yet been referenced specifically in any publications.