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The figures below are serial sections of human tonsil. IHC was carried out on paraffin-embedded sections following heat-mediated antigen retrieval using citric acid. Blocking was with 1% bovine serum albumin (BSA). Detection was via a horseradish peroxidase (HRP)-conjugated ABC system (goat anti-rabbit IgG H&L (ab207995) at 1:300 dilution) and 3,3′-diaminobenzidine (DAB) was used as the chromogen.
|Antibody||Leading anti-Ki67 polyclonal||Anti-Ki67 antibody (ab15580)|
|Staining pattern||Low specificity with high background||Specific with low background|
|Antibody||Leading anti-Ki67 monoclonal||Anti-Ki67 antibody [SP6] (ab16667)|
|Staining pattern||Specific with very low background||Specific with very low background|