A comparison of leading Ki67 antibodies for IHC

A comparison of anti ki67 antibodies for immunohistochemistry (IHC) with the leading alternatives by an independent academic collaborator.


  • Our anti-Ki67 antibodies accurately stain proliferating cells in tissue sections
  • They produce less background and can be used at much lower concentrations than the leading alternatives
  • We have knockout validated our anti-Ki67 antibodies used here to ensure specificity

The figures below are serial sections of human tonsil. IHC was carried out on paraffin-embedded sections following heat-mediated antigen retrieval using citric acid. Blocking was with 1% bovine serum albumin (BSA). Detection was via a horseradish peroxidase (HRP)-conjugated ABC system (goat anti-rabbit IgG H&L (ab207995) at 1:300 dilution) and 3,3′-diaminobenzidine (DAB) was used as the chromogen.

Polyclonal antibodies

AntibodyLeading anti-Ki67 polyclonalAnti-Ki67 antibody (ab15580)
Knockout validated?NoYes
Optimal dilution1:1001:600
Staining patternLow specificity with high backgroundSpecific with low background

Monoclonal antibodies

AntibodyLeading anti-Ki67 monoclonalAnti-Ki67 antibody [SP6] (ab16667)
Knockout validated?NoYes
Optimal dilution1:2001:1,000
Staining patternSpecific with very low backgroundSpecific with very low background

View the datasheets for our anti-Ki67 antibodies (ab15580 and ab16667) tested here

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