Mounting media and blocking sera for cell imaging

High-quality reagents to support your cell-imaging experiments, including mounting media and sera for blocking.​

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Mounting media

Fluoroshield is an aqueous mounting medium with a unique formulation that prevents rapid photobleaching of commonly used fluorescent labels during your experiment. In addition, fluorescence of the sample is retained during prolonged storage at 4°C in the dark.

CyGEL™ is a novel thermoreversible hydrogel that enables easy immobilization of live non-adherent cells and organisms, and their subsequent recovery after microscopy. CyGEL™ is liquid when cold and a gel at ~21°C. It is optically clear with low autofluorescence. For imaging experiments longer than 2 hours, we recommend CyGEL Sustain™, which is specially formulated to allow the addition of RPMI and similar culture media, enabling cells to survive for hours.

Explore Fluoroshield and CyGELTM products.

Sera for blocking

Most immunostaining protocols include a blocking step to reduce non-specific binding of the antibody. We offer high-quality sera from a number of different species, including goat, donkey, guinea pig, mouse and rabbit. Simply match the serum species to the species that the secondary antibody was raised in.  

Explore sera.

Selected references

Lambert, K.G.  et al. Contingency-based emotional resilience: effort-based reward training and flexible coping lead to adaptive responses to uncertainty in male rats. Front. Behav. Neurosci. 8:124 (2014).

Chung, C.Y.  et al. Progressive Proximal-to-Distal Reduction in Expression of the Tight Junction Complex in Colonic Epithelium of Virally-Suppressed HIV+ Individuals. PLoS Pathog. 10:e1004198 (2014).

Hernandez-Delgadillo, R.  et al. Bismuth oxide aqueous colloidal nanoparticles inhibit Candida albicans growth and biofilm formation. Int J Nanomedicine. 8:1645-52 (2013).

Guo, Y.  et al. PEG-Like Nanoprobes: Multimodal, Pharmacokinetically and Optically Tunable Nanomaterials. PLoS One. 9:e95406 (2014).

Najm, F.J.  et al. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol. 31:426-33 (2013).

Witasp, A.  et al. Elevated circulating levels and tissue expression of pentraxin 3 in uremia: a reflection of endothelial dysfunction. PLoS One. 8:e63493 (2013).

Dzinic, S.H.  et al. Identification of an intrinsic determinant critical for maspin subcellular localization and function. PLoS One. 8:e74502 (2013).