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Pharmacokinetics is the mathematics of the time course of absorption, distribution, metabolism, and excretion of drugs in the body. In an effort to examine the effectiveness of Anti-PEG RabMAb primary antibodies to track the metabolism rate of a PEGylated antigen within an animal model, the following experiment was performed.
1. A rat was injected intraperitineally using 100ug of
mouse IgG-PEG as an antigen.
2. Blood was drawn at 0.5, 1, 2, 4, 10, 24 and 48 hours post-injection.
3. The antiserum was extracted and tested for IgG concentrations
using Anti-PEG RabMAb primary antibody in an ELISA assay.
4. In a 96-well plate coated with 5ug/mL of Anti-PEG RabMAb primary
antibody (ab51257, clone: PEG-B-47), PEG-HRP and the antiserum
5. Using quantified IgG concentrations from the rat antiserum along with
a standard curve generated from an IgG-PEG + HRP-PEG competitive
assay (Fig. 1), the concentration of injected mouse IgG-PEG over time
can be determined (Fig. 2).
As demonstrated below, Anti-PEG RabMAb primary antibodies can be a used to accurately quantify targets of interest and investigate the pharmacokinetcs of PEGylated drugs or antigens within a system (Fig. 2).
Fig 1. Mouse IgG-PEG and PEG-HRP competitive ELISA assay using
varying amounts of IgG-PEG and PEG-HRP at a dilution factor of