Antibodies for in vitro and in vivo studies, including neutralization, blocking or activation/proliferation.
The presence of endotoxin can significantly impact both in vitro and in vivo experiments, causing non specific cellular responses and false readings in cell-based assays.
Endotoxin is an aggregate of acidic lipopolysaccharides (LPS) that is shed from bacteria and is a heat-stable ubiquitous contaminant. Although bacteria are often removed by using a 0.2 μm sterilizing grade filter, LPS contamination is difficult to eliminate via filtering or autoclaving. Limulus amoebocyte lysate (LAL) assays are the most commonly used endotoxin detection methods.
Explore our range of Low Endotoxin, Azide Free antibodies:
Endotoxin levels ≤ 1 EU/ml as determined by the LAL assay
Azide-free and carrier-free
Supplied at 1 mg/mL concentration
Quality tested in a range of applications, including flow cytometry and functional activity assays
Wu H et al.IFN-t Mediated Control of Bovine Major Histocompatibility Complex Class I Expression and Functionviathe Regulation of bta-miR-148b/152 in Bovine Endometrial Epithelial Cells.Front Immunol 9:167 (2018) PubMed ID: 29456541