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Small molecules and polymers are common components in medicine, food, cosmetics, pesticides, narcotics and research reagents. They can also be found in the human body and in the environment.
Antibodies against this class of antigen are increasingly used in:
Rabbits can generate a vigorous immune response against various small molecule haptens conjugated appropriately to immunogenic carrier proteins.
Fig. 1 - Generation of specific antibodies against small molecule haptens
With our RabMAb® platform a large panel of high affinity and specific monoclonal antibodies can be generated and selected according to your application needs.
Co-developed with UCSF researcher Dr. Kevan Shokat for protein kinase substrate profiling applications, this antibody provides:
Western blot analysis using Thiophosphate Ester Specific RabMAb primary antibody (ab92570)*. In vitro kinase reaction using JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S followed by alkylation with PNBM (2.5mM).
Kinase reaction was carried out for 30 minutes at room temperature. PNBM alkylation (2.5mM) was for 2 hours at room temperature.
Reaction products were analyzed by western blot with thiophosphate ester specific RabMAb primary antibody (ab92570) at 1:5000, a-GST antibody confirms equal loading. Note: PNBM also reacts with cysteine residues and may decrease the affinity of other antibody-antigen interactions.
Co-developed with Pantex, a division of Bio-Analysis, the anti-Progesterone RabMAb primary demonstrates:
Specificity for Progesterone vs other steroid hormones: