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IHC-validated recombinant antibodies for use with NanoString’s GeoMx® DSP Protein Barcoding Service

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  • Primary antibodies
    • Recombinant antibodies
      • Carrier-free antibodies
        • How we validate our antibodies
          • Our approach to IHC antibody validation
            • Carrier free IHC-validated products
              • NanoString
                • GeoMx® Digital Spatial Profiler
                  • Webinar: Morphology Driven High-Plex Spatial Analysis of Tissue Microenvironments

                    Get the best antibodies for digital spatial profiling through our collaboration with NanoString. 

                    Updated October 12th, 2022​​​

                    To profile the biology of a system it is critical to explore a broad set of protein and RNA markers. NanoString's GeoMx RNA and Protein Assays allow you to perform ultra high-plex, spatially resolved analysis with a single tissue slide. 
                    Abcam has partnered with NanoString to offer our range of IHC-validated, carrier-free recombinant monoclonal antibodies for use with the GeoMx Digital Spatial Profiler (DSP) Protein Barcoding Service. This gives you the flexibility to customize the GeoMx Protein Assay with over 5,000 protein targets.
                    Contact us to discuss your GeoMx DSP antibody needs. 
                    To learn more about GeoMx DSP technology, instrument, or sample profiling services please visit NanoString's website or contact NanoString.

                    To profile the biology of a system it is critical to explore a broad set of protein and RNA markers. NanoString's GeoMx RNA and Protein Assays allow you to perform ultra high-plex, spatially resolved analysis with a single tissue slide. 

                    Abcam has partnered with NanoString to offer our range of IHC-validated, carrier-free recombinant monoclonal antibodies for use with the GeoMx Digital Spatial Profiler (DSP) Protein Barcoding Service. This gives you the flexibility to customize the GeoMx Protein Assay with over 5,000 protein targets.

                    Contact us to discuss your GeoMx DSP antibody needs. 

                    To learn more about GeoMx DSP technology, instrument, or sample profiling services please visit NanoString's website or contact NanoString.


                    Overview

                    • IHC-validated antibodies suitable for Nanostring Protein Barcoding Services
                      • Robust IHC validation at Abcam
                      • Recombinant monoclonal antibodies
                      • Carrier-free antibody formulations
                    • How to order

                    IHC-validated recombinant antibodies for NanoString’s GeoMx Protein Barcoding Service

                    GeoMx Protein Assays, developed by NanoString, are high-plex cocktails of oligonucleotide-labeled antibodies bound to tissue sections using standard immunohistochemical (IHC) staining methods. Validated for use in multiplex applications, they enable you to profile many more markers than traditional methods.

                    • Find out more about how GeoMx Protein Assays can help your research.

                    Abcam’s highly specific and sensitive IHC-validated antibodies enable the best results with GeoMx Protein Assays in multiple ways.

                    Robust IHC validation 

                    Our antibodies undergo a variety of validation methods including staining multi-normal human tissue microarrays (TMAs), multi-tumor human TMAs, and rat/mouse TMAs during both development and QC.

                    Whenever possible our antibodies are knockout-validated and we are working to extend our range of KO-validated antibodies for IHC.

                    Recombinant monoclonal antibodies: infinitely better

                    Recombinant antibodies are manufactured to overcome the limitations of other manufacturing methods, like genetic drift, which can lead to variations in the final primary antibodies produced. Recombinant monoclonal antibodies give you: 

                    • High consistency, with high affinity across batches
                    • Improved sensitivity and confirmed specificity
                    • Reliable long-term supply for easy scalability

                    View all IHC-validated recombinant monoclonal antibodies

                    Conjugation-ready carrier-free recombinant monoclonal antibodies

                    Our recombinant antibodies are also available as carrier-free antibodies for a flexible and simple solution. The carrier-free format gives you:

                    • BSA, sodium azide, and glycerol-free format that supports consistent and successful conjugation. 
                    • 1mg/mL to eliminate the need to concentrate before conjugation
                    • Purified IgG to increase conjugation yield

                    View all IHC-validated carrier-free recombinant monoclonal antibodies

                    Image (above): Illustration of DSP experiment using a panel containing Abcam's recombinant rabbit monoclonal antibodies. Regions of interest (ROI) are identified using fluorescent dye-conjugated antibodies against CD3, CD20 and PanCK. Within each ROI, the degree of staining is identified for each oligo-conjugated antibody in the cocktail.

                    How to order

                    1. Contact Abcam to select your IHC-validated carrier-free antibody.  Once we understand your needs, we will provide a quotation with an estimated lead time. Alternatively, you can choose from our range of products. 
                    2. Place an order for >500ug of your chosen antibody with Abcam. We recommend validating your chosen clone by purchasing a smaller aliquot (eg 100ul) before placing your >500ug antibody order with us.
                    3. Get in contact with NanoString to initiate the GeoMx Protein Barcoding Service for your chosen antibody.

                    For more information about the GeoMx Protein Barcoding Service conjugation and QC processes contact NanoString. 

                    Contact Abcam to discuss your GeoMx DSP antibody needs


                    NanoString, nCounter and GeoMx are trademarks or registered trademarks of NanoString Technologies, Inc., in the United States and/or other countries.





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