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To profile the biology of a system, it is critical to explore a broad set of protein and RNA markers. NanoString's GeoMx RNA and Protein Assays allow you to perform high-plex, spatially resolved analysis of up to 96 protein or RNA targets with a single tissue slide.
Abcam has partnered with NanoString to provide custom GeoMx-formatted versions of our IHC-validated recombinant antibodies. This service offers researchers the flexibility to customize the GeoMx Protein Assays with up to five custom antibodies.
GeoMx Protein Assays use a high-plex cocktail of oligo-labeled antibodies, which are bound to tissue sections using standard immunohistochemical staining methods
Firstly, tissues are visualized with up to four additional fluorescent dye-labeled antibodies for selection of regions of interest (ROI) for profiling.
Then, oligonucleotide tags are decoupled from the antibodies from discrete ROI via UV light exposure. Released tags are aspirated with a micropipette without ever touching the tissue, dispensed in a 96-well plate and ultimately quantitated in a standard NanoString nCounter® assay. Finally, counts are mapped back to tissue location, yielding a spatially-resolved digital profile of protein abundance.
We offer a custom conjugation service to provide GeoMx-formatted recombinant rabbit monoclonal antibodies. Abcam will supply a panel of up to five GeoMx-formatted antibodies for spiking into your NanoString GeoMx Protein Assay cores or modules. We will also provide the Probe R reagent required to use in combination of every GeoMx-formatted antibody.
GeoMx Protein Assays require highly specific and sensitive IHC-validated such as Abcam's recombinant monoclonal antibodies. Using recombinant antibodies also ensures that performance is consistent, with no lot-to-lot variation.
Image (above): Illustration of DSP experiment using a panel containing Abcam's recombinant rabbit monoclonal antibodies. Regions of interest (ROI) are identified using fluorescent dye conjugated antibodies against CD3, CD20 and PanCK. Within each ROI, the degree of staining is identified for each oligo-conjugated antibody in the cocktail.
Once we understand your needs, we will provide a quotation with an estimated lead time. The oligo-conjugation service consumes a significant amount of carrier-free antibody. If we have sufficient antibody in stock in our US office, we expect a 5–6 week lead-time for US customers and 6–7 weeks for customers in Europe. If our conjugation service is especially busy, then the lead time may be 8–10 weeks for US customers and 9–11 weeks for customers in Europe. Lead times in other regions will vary.
The antibodies we have stock for are listed below. We are able to offer this service for all our recombinant antibodies, but if we do not have sufficient carrier-free antibody in stock, lead times may be longer.
Once your order is placed, we will process the antibody, by conjugating an oligonucleotide tag to it, so that it is suitable for use in a GeoMx Protein Assay. We will QC test the antibody to confirm successful oligo-conjugation. The oligo-conjugated antibody will be tested in conventional chromogenic IHC alongside the same unconjugated antibody to confirm that the specificity of the antibody has not changed. A complementary Probe R oligo will also be provided to use the GeoMx-formatted antibody in a GeoMx Protein Assay.
To learn how to incorporate the Abcam’s GeoMx formatted antibodies into your panel, please refer to the NanoString's DSP protocol book. If you are running the experiment in-house using a GeoMx DSP Profiler instrument, you will need a software widget from NanoString to use the Abcam antibody and Probe R. To obtain this, you should email NanoString at GeoMx@nanostring.com, sending them a spreadsheet that we will provide to you with the relevant code details.
Please contact us if your antibody of interest is not on this list.
NanoString, nCounter and GeoMx are trademarks or registered trademarks of NanoString Technologies, Inc., in the United States and/or other countries.