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By introducing a unique sequence of amino acids into a recombinant protein, antibodies against the corresponding epitope tag can help you track protein expression and visualize the protein in tissues, cells and organelles.
Epitope tag sequences and antibodies
|Myc tag||EQKLISEEDL||Myc tag antibodies|
|His tag||HHHHHH||His tag antibodies|
|HA tag||YPYDVPDYA||HA tag antibodies|
|VSV-G||YTDIEMNRLGK||VSV-G tag antibodies|
|V5 tag||GKPIPNPLLGLDST||V5 tag antibodies|
|T7 tag||MASMTGGQQMG||T7 tag antibodies|
|Glu-Glu tag||CEEEEYMPME||Glu-Glu tag antibodies|
|CBP tag||KRRNKKNFIAVSAA||CBP tag antibodies|
|S tag||-||S tag antibodies|
|DDDDK tag||DYDDDDK||DDDDK antibodies (equivalent to FLAG from Sigma)|
Anti-Myc tag (ab32), staining the Myc tag in HEK293 cells transfected with pCMV4-Myc-Mfn2 plasmid by ICC/IF.
|Reporter protein antibodies|
|GFP/YFP antibodies||GST antibodies|
|RFP antibodies||Luciferase antibodies|
|MBP antibodies||mCherry antibodies|
|FITC antibodies||Biotin antibodies|
|HRP antibodies||GAL4 antibodies|
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