Directly conjugated antibodies for immunofluorescence and flow cytometry
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Choose the best primary conjugates to develop your own multicolor immunofluorescence or flow cytometry panel.
Proteins rarely operate in isolation. Instead, they typically function within multi-subunit complexes. Targeting proteins in their multi-component state is essential to understand their function and to accurately inform the development of therapeutics.
Without the right set of tools, protein analysis can be complicated and generate misleading results. Cross-species reactivity can arise when using both primary and secondary antibodies (indirect detection), or there may be challenges in sourcing appropriately labeled primary conjugated antibodies (direct detection).
To overcome these issues, we have launched an exclusive range of directly conjugated antibodies with a variety of fluorescent labels.
Browse all conjugated recombinant antibodies
Why you should choose Abcam's primary conjugates:
- Recombinantly engineered to deliver the highest batch-to-batch consistency
- Conjugated to 9 Alexa Fluor® Dyes, FITC, PE, PerCP, APC, HRP, Biotin
- Flow conjugates tested in ICC/IF to confirm specific target binding
- Free dye removed to ensure a high signal-to-noise ratio
- Custom conjugation available for all recombinant clones (learn more)
| Label | Validated application(s) |
| Alexa Fluor ® dyes | Immunofluorescence (ICC/IF), immunohistochemistry (IHC), flow cytometry |
| Phycoerythrin (R-PE) | Flow cytometry (internally validated in ICC/IF) |
| FITC (Fluorescein isothiocyanate) | ICC/IF and/or flow cytometry |
| APC (Allophycocyanin) | Flow cytometry (internally validated in ICC/IF) |
| PerCP (Peridinin chlorophyll) | Flow cytometry (internally validated in ICC/IF) |
Explore our range of primary conjugates:
Download here the fluorochrome chart with the emission and excitation fluorescence spectra for 30 of the most commonly used fluorochromes.
| Label | Color* | ExMax (nm) | EmMax (nm) | EC | QY |
| AF405 | Violet | 402 | 421 | 35,000 | - |
| AF488 | Cyan-green | 495 | 519 | 73,000 | 0.92 |
| FITC | Cyan-green | 495 | 519 | 72,000 | 0.92 |
| AF555 | Yellow-green | 555 | 565 | 155,000 | 0.1 |
| R-PE | Yellow-green | 565 | 578 | 1,960,000 | 0.84 |
| AF568 | Orange | 578 | 603 | 88,000 | 0.69 |
| AF594 | Orange-red | 590 | 617 | 92,000 | 0.66 |
| APC | Far-red | 650 | 660 | 700,000 | 0.68 |
| AF647 | Far-red | 650 | 668 | 270,000 | 0.33 |
| PerCP | Far-red | 482 | 675 | 1,960,000 | - |
| AF680 | Near-IR | 679 | 702 | 183,000 | 0.36 |
| AF790 | Near-IR | 784 | 814 | 260,000 | - |
* = approximate color of the emission spectrum.
Abbreviations: AF = Alexa Fluor®. ExMax = Excitation max. EmMax = Emission max. EC = extinction coefficient (cm-1 M-1). QY = Quantum Yield.
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.