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Conjugated antibodies for immunofluorescence and flow cytometry

Related

  • Antibody conjugation solutions
    • View all conjugated primary antibodies
    • Conjugation-ready: carrier free antibody formulations
      • Antibody conjugation kits
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                                        • Direct vs. indirect immunofluroescence

                                          ​Less background, bright results 

                                          Over 3,000 fluorescent-labeled antibodies to develop your multiplex panel 

                                          Proteins rarely operate in isolation. Instead, they typically function within multi-subunit complexes. Targeting proteins in their multi-component state is essential to understand their function and to accurately inform the development of therapeutics.

                                          Without the right set of tools, protein analysis can be complicated and generate misleading results. Cross-species reactivity can arise when using both primary and secondary antibodies (indirect detection), or there may be challenges in sourcing appropriately labeled primary conjugated antibodies (direct detection). 

                                          To overcome these issues, we have launched an exclusive range of conjugated antibodies with a variety of fluorescent labels.

                                          Browse all conjugated antibodies for immunofluorescence

                                          Browse all conjugated antibodies for flow cytometry


                                          Why you should choose Abcam's conjugated antibodies

                                          • Recombinantly engineered to deliver the highest batch-to-batch consistency
                                          • Conjugated to Alexa Fluor® Dyes, FITC, PE, PerCP, APC, and tandem dyes;
                                          • Flow cytometry conjugates tested in ICC/IF to confirm specific target binding;
                                          • Free dye removed to ensure a high signal-to-noise ratio;
                                          • Custom antibody labeling services available - contact us to discuss your needs.


                                                                            LabelValidated application(s)
                                          Alexa Fluor ® ​dyesImmunofluorescence (ICC/IF), immunohistochemistry (IHC), flow cytometry
                                          Phycoerythrin (R-PE)Flow cytometry (internally validated in ICC/IF)
                                          FITC (Fluorescein isothiocyanate)ICC/IF and/or flow cytometry
                                          APC (Allophycocyanin)Flow cytometry (internally validated in ICC/IF)
                                          PerCP (Peridinin chlorophyll)Flow cytometry (internally validated in ICC/IF)

                                          Learn more about our complete range of antibody conjugation solutions.

                                          Explore today our range of conjugated antibodies

                                          Download our fluorophore table with the ​​emission and excitation fluorescence spectra for the most commonly used fluorochromes.​

                                          LabelColor*ExMax (nm)EmMax (nm)QY
                                          AF405Violet402421-
                                          AF488Cyan-green4955190.92
                                          FITCCyan-green4955190.92
                                          AF555Yellow-green5555650.1
                                          R-PEYellow-green5655780.84
                                          AF568Orange5786030.69
                                          AF594Orange-red5906170.66
                                          APCFar-red6506600.68
                                          AF647Far-red6506680.33
                                          PerCPFar-red482675-
                                          AF680Near-IR6797020.36
                                          AF790Near-IR784814-
                                          Browse all conjugated recombinant antibodies

                                          * = approximate color of the emission spectrum.

                                          Abbreviations: AF = Alexa Fluor®. ExMax = Excitation max. EmMax = Emission max. EC = extinction coefficient (cm-1 M-1). QY = Quantum Yield.

                                          Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies. 





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