Directly conjugated antibodies for immunofluorescence and flow cytometry
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Offering reliable and specific research tools for multicolor immunofluorescence and flow cytometry.
Proteins rarely operate in isolation but rather function within multi-subunit complexes. It has now become essential to study proteins in their multi-component state rather than individually.
Without the right set of tools, protein analysis can be extremely difficult; cross-species reactivity can arise when using both primary and secondary antibodies (indirect detection). There may be challenges in sourcing appropriately labeled conjugated primary antibodies (direct detection).
To overcome these issues, we have launched an exclusive range of directly conjugated antibodies with a variety fluorescent labels.
Benefits include:
- Use of RabMAb® technology – recombinant antibody generation, which provides high antibody affinity and specificity
- Removal of free dye following conjugation, ensuring a high signal to noise ratio
- In-house manufacturing within our laboratories to ensure high-quality conjugates
- Supporting data including images, references, and customer reviews
- Thorough validation in multiple applications, including batch-validation
Fluorescent-labeled primary antibodies:
| Label | Validation data |
| Alexa Fluor® | Immunofluorescence (ICC/IF), immunohistochemistry (IHC), flow cytometry |
| Phycoerythrin (R-PE) | ICC/IF and/or flow cytometry |
| FITC (Fluorescein isothiocyanate) | ICC/IF and/or flow cytometry |
| APC (Allophycocyanin) | ICC/IF and/or flow cytometry |
| PerCP (Peridinin chlorophyll) | ICC/IF and/or flow cytometry |
Learn more about our most popular conjugates:
| Label | Color* | ExMax (nm) | EmMax (nm) | EC | QY |
| AF405 | Violet | 402 | 421 | 35,000 | - |
| AF488 | Cyan-green | 495 | 519 | 73,000 | 0.92 |
| FITC | Cyan-green | 495 | 519 | 72,000 | 0.92 |
| AF555 | Yellow-green | 555 | 565 | 155,000 | 0.1 |
| R-PE | Yellow-green | 565 | 578 | 1,960,000 | 0.84 |
| AF568 | Orange | 578 | 603 | 88,000 | 0.69 |
| AF594 | Orange-red | 590 | 617 | 92,000 | 0.66 |
| APC | Far-red | 650 | 660 | 700,000 | 0.68 |
| AF647 | Far-red | 650 | 668 | 270,000 | 0.33 |
| PerCP | Far-red | 482 | 675 | 1,960,000 | - |
| AF680 | Near-IR | 679 | 702 | 183,000 | 0.36 |
| AF790 | Near-IR | 784 | 814 | 260,000 | - |
* = approximate color of the emission spectrum.
Abbreviations: AF = Alexa Fluor®. ExMax = Excitation max. EmMax = Emission max. EC = extinction coefficient (cm-1 M-1). QY = Quantum Yield.
ab196158: Recombinant rabbit monoclonal anti-Calreticulin (Alexa Fluor® 488) antibody (green), staining Calreticulin in wild-type HAP1 cells (top panel) and Calreticulin-knockout HAP1 cells (bottom panel). See the datasheet for more details. See more information on KO-testing here.
ab209960: Recombinant rabbit monoclonal anti-PD-L1 (Alexa Fluor® 647) antibody staining PD-L1 in CHO (blue line) and CHO-PD-L1 (red line) transfected cells. See the datasheet for more details.
Browse all conjugated recombinant antibodies
We also offer a custom conjugation service. For more information click here.
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.