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With the advent of fluorescent western blotting, multiple proteins can now be analyzed simultaneously using different fluorophores.This growing technique has been proven to provide improved linearity and increased reproducibility when compared to standard chemiluminescent detection methods in Western blot.
Fluorescent western blotting works optimally in the near-infrared region of the spectrum in order to avoid the chance of membrane autofluorescence within the visible light range.
Therefore, this technique calls for bright and stable near infrared dyes, such as Alexa Fluor® 680 and Alexa Fluor® 790.
ab184577 - Anti-alpha Tubulin antibody [DM1A] (Alexa Fluor® 790):
We have now conjugated our most popular loading controls to Alexa Fluor® 680 and Alexa Fluor® 790 dyes specifically for this emerging application.
This offering opens the opportunity for researchers to analyze their western blot data with the brightness and consistency of Alexa Fluor® dyes and the reliability of Abcam's best loading controls.
ab184092 - Anti-beta Actin antibody [mAbcam 8226] (Alexa Fluor® 680):
Alexa Fluor® labeled loading controls:
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.