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High-resolution imaging allows you to track the location and movement of proteins within the cellular environment. To ensure proper interpretation of these experiments, it is necessary to confirm whether the protein is actually located in the subcellular environment we expect.
Two approaches can be used to confirm the subcellular localization of a protein: organelle-specific antibodies and organelle stains.
Organelle stains can be used as counterstains to help identify the location of specific proteins and targets of interest within the cell, while antibodies against proteins associated with a specific organelle can lead to a better understanding of cellular function.
For nuclear staining, you have several options, including the highly success DRAQ staining.
|Over 60 targets for multiple organelles and structures||Easily identify cellular organelles and structures|
|The brightest dyes available||Greater sensitivity with Alexa Fluor® dyes|
|Multiple host species and clonalities||Easily study multiple targets with RabMAb® reagents|
|Use in live and fixed cells||Easy to implement in your current staining protocols|
|Suitable for proliferating and non-proliferating cells||May be used in most cellular or tissue samples|
|High photostability||Minimal photobleaching allows longer exposure times|
When might a Cytopainter dye work best for you?
DRAQ5™ and DRAQ7™ are far-red fluorescent dyes that are used for nuclear staining.
DRAQ5™ is suitable for use on a wide range of instrumentation platforms and can be simply integrated into existing protocols and procedures. Applications of DRAQ5™ include:
DRAQ7 dyes only stain nuclei in dead or permabilized cells and does not enter intact live cells.
Sample: Methanol fixed Hek293 cells
Green staining: Subcellular marker ab197496 - Mouse monoclonal to alpha 1 Sodium Potassium ATPase (Alexa Fluor® 488)
Blue staining: Nucleus labeled with DAPI
Sample: whole Hydractinia fixed in 4% PFA
Red staining: Actin filaments stained with CytoPainter F-actin staining kit - Red Fluorescence (ab112127) at 1:500 dilution
Blue staining: Nucleus labeled with Hoechst nuclear staining