Carrier-free formulations for recombinant RabMAb® antibodies

​Recombinant rabbit monoclonal antibodies available in carrier-free formats for easy antibody-labeling and functional assays, including mass cytometry and multiplex IHC, as well as antibody pairs for sandwich immunoassays.

​Standard RabMAb® rabbit monoclonal antibodies are stored in a solution phosphate buffered saline (PBS) with several key carriers: bovine serum albumin (BSA), glycerol, and sodium azide. 

While BSA and azide are essential components for maintaining antibody stability and preventing antibody contamination, these components can also hinder effective conjugation to fluorochromes, metals, and enzymes.

BSA competes with the primary antibody to attach to the label of interest, which greatly reduces the conjugation efficiency. The presence of sodium azide in the antibody solution can be toxic to cells, limiting the antibody's use in cell culture. In addition, sodium azide can interfere with antibody conjugation and inhibits the activity of horseradish peroxidase (HRP).

To give you the flexibility you need, we've reformulated a number of our antibodies to be carrier-free, that is, BSA and azide-free, making them ideal for easy antibody labeling and functional assays.

View all carrier-free recombinant RabMAb® antibodies

View all carrier-free antibody pairs


Features

  • BSA-free, sodium azide-free, and glycerol-free
  • Supplied at higher concentration (1 mg/mL in 100 µg size)
  • Ready for conjugation to fluorochromes, metals, and enzymes
  • Ready format for mass cytometry (eg CyTOF ®) and multiplex IHC
  • Low endotoxin-tested (selected products)
  • Custom formulation, conjugation and bulk orders available
    Custom formulation, conjugation and bulk orders available 


Applications

These reformulated carrier-free recombinant rabbit monoclonal antibodies are ideal for many demanding applications, including multiplexed single-cell analysis and imaging (mass cytometry, imaging mass cytometry, multiplex IHC).

Mass cytometry (such as CyTOF ®) is a novel flow cytometry platform that uses metal-conjugated antibodies to characterize and quantify more than 40 parameters simultaneously at the single cell level. 

Imaging Mass CytometryTM combines the advantages of metal-conjugated antibodies and immunohistochemical/immunocytochemical methods to study cell-cell interactions at the subcellular resolution and delineate tumor heterogeneity.


Carrier-free antibody pairs for immunoassays

  • All include a recombinant capture and detector antibody pair, suitable for ELISA-based assays.
  • Offer even more flexibility in terms of conjugation and assay set-up
  • Guarantee specific results: our pairs are screened in plasma and serum to ensure specificity in complex samples
  • Available in 10 x 96 plates


View all carrier-free recombinant RabMAb® antibodies

View all carrier-free antibody pairs





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