Carrier-free formulations for recombinant antibodies
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Discover a range of recombinant monoclonal antibodies and matched antibody pairs available in carrier-free formats
Updated March 15, 2023
To enhance flexibility, we've formulated over 10,000 of our antibodies and matched antibody pairs to be carrier-free and conjugation-ready.
Carrier-free antibodies are specifically designed for antibody labeling, saving time with a simplified protocol. The antibody is added directly to the conjugation reaction, avoiding antibody loss without the need to perform buffer exchange or concentration.
By combining carrier-free antibodies with our Lightning-Link® kits, conjugation has never been easier.
View all carrier-free recombinant antibodies
View all carrier-free matched antibody pairs
View all carrier-free capture and detector antibodies
Contents
- Benefits of our carrier-free antibodies and matched antibody pairs
- Low endotoxin, azide-free bioactive antibodies
- Carrier-free antibodies in multiplex assays
- Carrier-free matched antibody pairs for ELISA-based assays and high-throughput screening (HTS
- View carrier-free reagents relevant to your research area
Benefits of our carrier-free antibodies and matched antibody pairs
| Carrier-free antibodies | Carrier-free matched antibody pairs |
| BSA, sodium azide, and glycerol-free for consistent conjugation | |
| Compatible with a variety of different labels such as fluorochromes, metal isotypes, oligonucleotides, and enzymes | |
| Recombinantly manufactured for the highest consistency between batches | |
| 1 mg/mL concentration, eliminating the need to concentrate prior to conjugation | Individual, unconjugated capture and detector antibodies are available in 100μg or 1mg size for easy scalability |
| Clone-validated in relevant applications such as IHC and flow cytometry | Screened in plasma and serum to ensure specificity in complex samples |
| Purified IgG to increase conjugation yield | Flexibility for seamless integration into your experiment |
Standard rabbit monoclonal antibodies are typically stored in a phosphate-buffered saline (PBS) solution with carrier proteins like bovine serum albumin (BSA), and other additives such as glycerol and sodium azide.
These components can hinder effective conjugation to fluorochromes, metals, oligonucleotides, and enzymes, so removing these proteins and additives enhances labeling.
- BSA competes with the primary antibody for the label of interest, which greatly reduces the conjugation efficiency
- Sodium azide in the antibody solution can be toxic to cells, limiting the antibody's use in cell culture
- Sodium azide can also interfere with antibody conjugation and inhibits the activity of horseradish peroxidase (HRP)
Endotoxin is an aggregate of acidic lipopolysaccharides (LPS) that is shed from bacteria and is a heat-stable ubiquitous contaminant. Although bacteria are often removed with a 0.2 μm sterilizing grade filter, LPS contamination is difficult to eliminate via filtering or autoclaving. The presence of endotoxin can significantly impact both in vitro and in vivo experiments, causing non-specific cellular responses and false readings in cell-based assays.
Our low endotoxin antibodies are designed for in vitro and in vivo studies, including neutralization, blocking, or activation/proliferation.
Benefits of our low endotoxin, azide-free bioactive antibodies:
Endotoxin levels ≤ 1 EU/ml, as determined by the LAL assay
Sodium azide, BSA and glycerol-free
Supplied at 1 mg/mL concentration
Quality tested in a range of applications, including flow cytometry and functional activity assays
Bulk sizes and custom concentrations available
Carrier-free antibodies in multiplex assays
Our carrier-free formats are ideal for building multiplex assays with your own panel of conjugated antibodies.
Flow based-assays:
Cytometry by Time Of Flight (CyTOF®) is a novel flow cytometry platform that uses metal-conjugated antibodies to characterize and quantify more than 40 parameters simultaneously at the single-cell level.
Multiplex IHC assays:
Imaging mass cytometry™ (IMC) combines the advantages of metal-conjugated antibodies and immunohistochemical/immunocytochemical methods to study cell-cell interactions at the subcellular resolution and delineate tumor heterogeneity.
Cyclic immunofluorescence (CyCIF) involves repeatedly staining and imaging slides, making it a reliable and inexpensive method for multiplex imaging.
Imaging Mass Cytometry™, IMC™, CyTOF® are trademarks of Fluidigm Canada Inc.
Carrier-free matched antibody pairs for ELISA-based assays and high-throughput screening (HTS)
In addition to ELISA assays, our carrier-free matched antibody pairs are ideal for non-conventional ELISA-based assays requiring different platforms, including high-throughput fluorescent proximity assays.
To enable complete flexibility, we offer over 1,000 antibody pairs and their individual capture and detector antibodies in carrier-free and customizable formats, so you can adapt your pair to your HTS assay.
View carrier-free reagents relevant to your research area
You can easily filter our range of carrier-free reagents to view targets for your research area with our search filters:
1. Click one of the options below to see our full ranges of carrier-free recombinant antibodies, matched antibody pairs, or capture and detector antibodies:
View all carrier-free, recombinant antibodies
View all carrier-free matched antibody pairs
View all carrier-free capture and detector antibodies
2. Scroll down until you see the ‘Research areas’ filter on the left-hand side
3. Choose your research area of interest from the list to find highly validated carrier-free reagents to your key targets