Carrier-free formulations for recombinant antibodies

To enhance flexibility in your workflow, we've formulated over 10,000 of our antibodies and matched antibody pairs to be carrier-free, or, BSA and azide-free, making them ideal for easy antibody labeling and functional and cell-based assay development. 

Standard rabbit monoclonal antibodies are typically stored in a phosphate-buffered saline (PBS) solution with carrier proteins like bovine serum albumin (BSA), and other additives such as glycerol and sodium azide.   

However, these components can hinder effective conjugation to fluorochromes, metals, oligonucleotides, and enzymes, affecting experiments requiring conjugated reagents. 

View all carrier-free recombinant antibodies

View all carrier-free matched antibody pairs

View all carrier-free capture and detector antibodies

Contents

• Carrier-free antibodies
  • Benefits of our carrier-free antibodies
  • Applications
• Carrier-free matched antibody pairs
  • Benefits of our carrier-free matched antibody pairs
  • Applications
• View carrier-free reagents relevant to your research area

Carrier-free antibodies 

Carrier-free antibodies provide a flexible, simple solution for your research needs.

Benefits of our carrier-free antibodies
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• BSA, sodium azide, and glycerol-free for consistent conjugation
• 1 mg/mL concentration which eliminates the need to concentrate prior to conjugation
• Purified IgG to increase conjugation yield
• Compatible with a variety of different labels such as fluorochromes, metal isotypes, oligonucleotides, and enzymes
• Clone validated in relevant applications such as IHC and flow cytometry
• Recombinantly manufactured to give you the highest level of consistency between batches with a scalable, secure supply

Applications

Conjugation

Carrier-free antibodies are specifically designed for antibody labeling and are conjugation ready:

• BSA competes with the primary antibody for the label of interest, which greatly reduces the conjugation efficiency
• Sodium azide in the antibody solution can be toxic to cells, limiting the antibody's use in cell culture
• Sodium azide can also interfere with antibody conjugation and inhibits the activity of horseradish peroxidase (HRP)

By using carrier-free antibodies, you can avoid antibody loss without the need to perform buffer exchange or concentration, saving you time with a simplified protocol. Simply add the antibody directly into the conjugation reaction.

Find out more about our comprehensive range of antibody conjugation solutions. 

Multiplex assays 

Our carrier-free formats are ideal for building multiplex assays with your own panel of conjugated antibodies. These technologies include flow-based assays (eg Cytometry by Time Of Flight [CyTOF^®]) or multiplex IHC assays (eg Imaging Mass Cytomtery™ [IMC™], and Cyclic Immunofluorescence [CycIF]).

CyTOF^®  (Cytometry by Time Of Flight) is a novel flow cytometry platform that uses metal-conjugated antibodies to characterize and quantify more than 40 parameters simultaneously at the single-cell level. 

View all CyTOF^®-ready formats

Imaging Mass Cytometry™ combines the advantages of metal-conjugated antibodies and immunohistochemical/immunocytochemical methods to study cell-cell interactions at the subcellular resolution and delineate tumor heterogeneity.

View all IMC™-ready formats 

Imaging Mass Cytometry™, IMC™, CyTOF^® are trademarks of Fluidigm Canada Inc.​​

• BSA, sodium-azide, and glycerol-free for consistent conjugation
• Screened in plasma and serum to ensure specificity in complex samples
• Recombinantly manufactured capture and detector pairs for the highest consistency between batches
• Flexibility for seamless integration into your experiment
• Individual, unconjugated capture and detector antibodies are available in a 100μg or 1mg size so you can easily scale up your experiment

Our carrier-free pairs are conjugation-ready and ideal for non-conventional ELISA-based assays requiring different platforms.

Applications

ELISA-based assays and high-throughput screening (HTS)

Our carrier-free pairs are conjugation-ready and ideal for non-conventional ELISA-based assays requiring different platforms.

In addition to ELISA assays, our matched antibody pairs can be used in other assays adapted to high-throughput scale such as fluorescent proximity assays. To enable complete flexibility, we offer over 900 antibody pairs and their individual capture and detector antibodies in carrier-free and customizable formats, so you can adapt your pair to your HTS assay. 

View all carrier-free matched antibody pairs

View all carrier-free capture and detector antibodies

View carrier-free reagents relevant to your research area

You can easily filter our range of carrier-free reagents to view targets for your research area with our search filters: 

1. Click one of the options below to see our full ranges of carrier-free recombinant antibodies, matched antibody pairs, or capture and detector antibodies: 

View all carrier-free, recombinant antibodies 

View all carrier-free matched antibody pairs 

View all carrier-free capture and detector antibodies 

2. Scroll down until you see the ‘Research areas’ filter on the left-hand side 

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3. Choose your research area of interest from the list to find highly validated carrier-free reagents to your key targets