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Recombinant rabbit monoclonal (RabMAb®)antibodies available in BSA and azide-free formats for easy antibody-labeling and functional assays, including mass cytometry and multiplex IHC.
Standard RabMAb® antibodies are stored in a solution containing phosphate buffered saline (PBS), bovine serum albumin (BSA), glycerol, and sodium azide.
While BSA and azide are essential components for maintaining antibody stability and preventing antibody contamination, these components can also hinder effective conjugation to fluorochromes, metals and enzymes.
BSA competes with the primary antibody to attach to the label of interest, which greatly reduces the conjugation efficiency. The presence of sodium azide in the antibody solution can be toxic to cells, limiting the antibody's use in cell culture. In addition, sodium azide can interfere with antibody conjugation and inhibits the activity of horseradish peroxidase (HRP).
We provide both recombinant rabbit monoclonal antibodies which have been reformulated to overcome these problems and improve conjugation effieciency, as well as convenient kits to remove BSA and azide.
These reformulated recombinant rabbit monoclonal antibodies are ideal for many demanding applications, including multiplexed single-cell analysis and imaging (mass cytometry, imaging mass cytomery, multiplex IHC).
Mass cytometry (such as CyTOF ®) is a novel flow cytometry platform that uses metal-conjugated antibodies to characterise and quantify more than 40 parameters simultaneously at the single cell level.
Imaging mass cytometry combines the advantages of metal-conjugated antibodies and immunohistochemical/immunocytochemical methods to study cell-cell interactions at subcellular resolution and delineate tumour heterogeneity.
PBS-only Recombinant RabMAb® antibodies are:
ab209897: Recombinant rabbit monoclonal anti-Ki67 antibody (green), staining Ki67 in wild-type HAP1 cells (top panel) and Ki67-knockout HAP1 cells (bottom panel).
ab199376: Rabbit monoclonal IgG - Isotype control (black) utilized as a negative control in flow cytometry analysis of fixed Jurkat cells. ab199376 shows no signal overlap.