Comparing PD-L1 antibody clones

A comparison of monoclonal antibody clones against PD-L1

In order to give you the best chance of finding the optimal PD-L1 clone for your research assay, you can now get access to four key recombinant monoclonal PD-L1 antibody clones from Abcam (73-10, 28-8, SP142 and CAL10), as well as the anti-mouse recombinant rabbit monoclonal EPR20529 and the mouse monoclonal PDL1/2746.

PD-L1 background

PD-L1 (also known as B7-H1, CD274) is a ligand that binds to PD-1 (programmed cell death-1), expressed on activated T cells, to evade anti-tumor responses. PD-L1 plays a role in inhibiting T cell activation and proliferation and has emerged as an important target in cancer treatment. PD-L1 protein detection by immunohistochemistry (IHC) testing is widely used as a predictive biomarker assay for anti–PD-1/PD-L1 therapies (additional details  here).

It is therefore vital to have reliable and reproducible tools to detect and measure PD-L1. You can now access all 4 anti-human recombinant rabbit monoclonal antibody clones in our trial size panel kit.

Table 1. A comparison of our PD-L1 primary antibody clones. These include Clone 28-8Clone 73-10Clone SP142Clone EPR20529Clone CAL10Clone PDL1/2746. Click on any of these links to view the relevant datasheet and purchase information. 

Independent PD-L1 testing

Independent studies have compared the performance of IVD grade/diagnostic PD-L1 IHC assay kits. The blueprint phase 2 project used the IVD grade/diagnostic IHC assays 73-10 (Dako/Agilent), 28-8 (Dako) and SP142 (Ventana), and the Karnik paper used 22C3 (Dako), SP263 (Ventana) and CAL10 (BioCare). See the links below to read the findings.

PD-L1 Immunohistochemistry Comparability Study in Real-Life Clinical Samples: Results of Blueprint Phase 2 Project

Immunohistochemical staining for programmed cell-death ligand 1 (PD-L1) in malignant thymoma and thymic carcinoma

PD-L1 in breast cancer: comparative analysis of 3 different antibodies

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