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A comparison of monoclonal antibody clones against PD-L1
For the best chance of finding the optimal PD-L1 clone for your research assay, you can now get access to four key recombinant monoclonal PD-L1 antibody clones from Abcam (73-10, 28-8, SP142, CAL10 and EPR19759), as well as the anti-mouse recombinant rabbit monoclonal EPR20529 and EPR23546-160.
Table 1. A comparison of PD-L1 primary antibody clones
Clones | 28-8 | 73-10 | SP142 | EPR19759 | CAL10 | EPR20529 | EPR23546-160 |
---|---|---|---|---|---|---|---|
Recombinant | Yes | Yes | Yes |
| Yes | Yes | Yes |
Epitope | Extracellular | Intracellular | Intracellular | Intracellular | Intracellular | Extracellular | Extracellular |
Host/Clonality | Rabbit mono | Rabbit mono | Rabbit mono | Rabbit mono | Rabbit mono | Rabbit mono | Rabbit mono |
Species reactivity | Human | Human | Human | Human | Human | Mouse | Mouse |
Applications verified | IHC-P, ICC/IF, Flow Cyt, WB | IHC-P, WB, ICC/IF, Flow Cyt (intra), IP | IHC-P, ICC/IF | Flow Cyt (intra), WB, IHC-P, ICC/IF, IP | IHC-P, ELISA, ICC/IF, WB, IP | ICC/IF, IP, WB | Flow Cyt, ICC/IF |
Ideal usage | IHC autostainer | IHC autostainer | ICC human, IHC autostainer | ICC human, IHC autostainer | IHC autostainer | ICC mouse | Flow Cytometry mouse |
PD-L1 (also known as B7-H1, CD274) is a ligand that binds to PD-1 (programmed cell death-1), expressed on activated T cells, to evade anti-tumor responses. PD-L1 plays a role in inhibiting T cell activation and proliferation and has emerged as an important target in cancer treatment. PD-L1 protein detection by immunohistochemistry (IHC) testing is widely used as a predictive biomarker assay for anti–PD-1/PD-L1 therapies.
It is therefore vital to have reliable and reproducible tools to detect and measure PD-L1. You can now access all four anti-human recombinant rabbit monoclonal antibody clones in our panel kit.
Independent PD-L1 testing
Independent studies have compared the performance of IVD grade/diagnostic PD-L1 IHC assay kits. The blueprint phase 2 project used the IVD grade/diagnostic IHC assays 73-10 (Dako/Agilent), 28-8 (Dako) and SP142 (Ventana), and the Karnik paper used 22C3 (Dako), SP263 (Ventana) and CAL10 (BioCare). See the links below to read the findings.
Immunohistochemical staining of lung cancer tissues with PD-L1 monoclonal antibody clones.