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Recombinant multiclonal antibodies are a defined mixture of carefully selected individual recombinant monoclonal antibodies designed to recognize different epitopes on the same antigen.
They are an ideal solution for applications where a polyclonal antibody would traditionally be used and are able to provide the specificity and reproducibility only available from a recombinant antibody.
The mix of antibodies that make up each one of the recombinant multiclonal antibodies is carefully controlled and consistent between batches. In addition, each recombinant monoclonal antibody included in the mix is developed from a defined set of genes that encode the antibodies with the desired characteristics. These features ensure consistency between batches, giving your work the long-term reproducibility it demands.
Because recombinant multiclonal antibodies bind to multiple epitopes on the same target protein, they provide excellent sensitivity, making them suitable for when you need a reagent capable of analyzing low-abundance targets or detecting multiple post-translational modifications at once.
Figures 1, 2, and 3 below compare the sensitivity of a polyclonal and recombinant multiclonal antibody for targets CD133, histone H3 (citrulline R2 + R8 + R17), and GFAP.
Figure 2. Our recombinant anti-Histone H3 (citrulline R2 + R8 + R17) multiclonal antibody [RM1001], ab281584 (left), tested in multiple sample types in western blot against our polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) antibody, ab5103.
Figure 3. Immunofluorescent analysis of mouse primary neural/glia cells labeling GFAP with our recombinant anti-GFAP multiclonal antibody, ab278054 (left), against our polyclonal anti-GFAP antibody, ab7260.