Von Willebrand Factor – our top endothelial marker
Read about the success of our Von Willebrand Factor antibody
Von Willebrand Factor antibody (ab6994) datasheet
The von Willebrand factor (VWF) glycoprotein is a useful marker for endothelial cells. It functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It participates in platelet-vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. In endothelial cells that produce VWF, the factor is stored in so-called Weibel-Palade bodies.
IF - Von Willebrand Factor antibody (ab6994)
Von Willebrand Factor antibody (ab6994) is one of our best-selling antibodies. It has been shown to cross-react with guinea pig, human, mouse and rat tissues and has been tested in the following applications:
We used this antibody to identify vascular structure in both human and mouse tissue. For human tisuue this antibody has very high affinity and can be used at 1:1000 to 1:2000 dilution, for mouse tissue it is around 1:500 to 1:800. This is a very specific antibody, and works well in formalin fixed paraffin embedded samples according to the protocol recommended by Abcam.
Abreview byBetty Mastropaolosubmitted 21 August 2004
Using this antibody to stain murine endothelial cells, I got gorgeous fluorescence staining at 1:500 dilution on fresh cells, either growing on a slide or dissociated. I predict that FACS analysis would also work well.
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The antibody has also been used in several papers as an endothelial cell marker:
Au-Yeung KKet al.Hyperhomocysteinemia activates nuclear factor-kappaB in endothelial cells via oxidative stress.Circ Res94:28-36 (2004).PubMed: 14630727
Liao Q et al. Down-regulation of the dual-specificity phosphatase MKP-1 suppresses tumorigenicity of pancreatic cancer cells. Gastroenterology 124:1830-45 (2003).PubMed: 12806617
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