Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5671] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671]
See all PRK3+PRK2+PRK1 primary antibodies
DescriptionRabbit monoclonal [EPR5671] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
SpecificityThis antibody only detects PKN3 phosphorylated at Threonine 718, PKN2 phosphorylated at Threonine 816, and PKN1 phosphorylated at Threonine 774.
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues surrounding Threonine 718 of Human PKN3 (Q6P5Z2), Threonine 816 of Human PKN2 (Q16513), and Threonine 774 of Human PKN1 (Q16512) proteins.
- HeLa cell lysates and Hek 293 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 104, 112, 99 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
All lanes : Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709) at 1/1000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Okadaic acid and Calyculin A.
Lysates/proteins at 10 µg per lane.
Predicted band size: 104, 112, 99 kDa
Bottom blot was probed with an anti-PNK2 antibody.
ab124709, at 1/10, staining PKN1 (phospho T774) + PKN2 (phospho T816) + PKN3 (phospho T718) in permeabilized Hek 293 cells by Flow cytometry.
Green: untreated cells
Red: Okadaic Acid + Calyculin A-treated cells
Blue: untreated cells preincubated with the immunizing phosphopeptides
Orange: Okadaic Acid + Calyculin A-treated cells preincubated with the immunizing phosphopeptides .
ab124709 has been referenced in 1 publication.
- Uehara S et al. Protein kinase N3 promotes bone resorption by osteoclasts in response to Wnt5a-Ror2 signaling. Sci Signal 10:N/A (2017). PubMed: 28851822