Overview

  • Product name

    Anti-PRKACG antibody [EP2647Y]
  • Description

    Rabbit monoclonal [EP2647Y] to PRKACG
  • Host species

    Rabbit
  • Specificity

    This antibody is likely to react with the alpha subunit, as it possesses 85.7% similar homology with the immunogen. Additionally, it may react with the beta subunit, possessing 78.6% homology.
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PRKACG (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, MCF7, PC3 and A549 cell lysates
  • General notes

     This product was previously labelled as PKA gamma (catalytic subunit)

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab108385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
    • Tissue specificity

      Testis specific. But important tissues such as brain and ovary have not been analyzed for the content of transcript.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links

    • Alternative names

      • cAMP-dependent protein kinase catalytic subunit gamma antibody
      • KAPCG_HUMAN antibody
      • KAPG antibody
      • PKA C gamma antibody
      • PKA C-gamma antibody
      • PKACg antibody
      • PRKACG antibody
      • Protein kinase cAMP dependent catalytic gamma antibody
      • Serine (threonine) protein kinase antibody
      see all

    Images

    • All lanes : Anti-PRKACG antibody [EP2647Y] (ab108385) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : PC3 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa

    References

    This product has been referenced in:

    • Yin C  et al. PID1 alters the antilipolytic action of insulin and increases lipolysis via inhibition of AKT/PKA pathway activation. PLoS One 14:e0214606 (2019). Read more (PubMed: 30990811) »
    • Cao W  et al. Hoxa5 Promotes Adipose Differentiation via Increasing DNA Methylation Level and Inhibiting PKA/HSL Signal Pathway in Mice. Cell Physiol Biochem 45:1023-1033 (2018). Read more (PubMed: 29439250) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

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    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T cell line)
    Gel Running Conditions
    Reduced Denaturing (12 % gel)
    Loading amount
    2e+006 cells
    Specification
    HEK293T cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Jan 29 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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