Overview

  • Product name

    Anti-PRKAG3 antibody [1C5D10]
    See all PRKAG3 primary antibodies
  • Description

    Mouse monoclonal [1C5D10] to PRKAG3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PRKAG3 aa 9-151. Expressed in E. coli.
    Database link: Q9UGI9

  • Positive control

    • WB: Recombinant human PRKAG3 protein (amino acids 9-151). PRKAG3 (AA: 9-151) - hIgGFc transfected HEK-293 cell lysate.
  • General notes

     This product was previously labelled as AMPK gamma 3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab233561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000.

Target

  • Function

    AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
  • Tissue specificity

    Skeletal muscle, with weak expression in heart and pancreas.
  • Sequence similarities

    Belongs to the 5'-AMP-activated protein kinase gamma subunit family.
    Contains 4 CBS domains.
  • Domain

    The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
    The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP.
  • Post-translational
    modifications

    Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5 AMP activated protein kinase subunit gamma 3 antibody
    • 5''-AMP-activated protein kinase subunit gamma-3 antibody
    • AAKG3_HUMAN antibody
    • AMPK gamma 3 chain antibody
    • AMPK gamma3 antibody
    • AMPK subunit gamma-3 antibody
    • AMPKG3 antibody
    • PRKAG3 antibody
    • Protein kinase AMP activated gamma 3 non catalytic subunit antibody
    see all

Images

  • All lanes : Anti-PRKAG3 antibody [1C5D10] (ab233561) at 1/500 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : PRKAG3 (amino acids 9-151) - hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
  • Anti-PRKAG3 antibody [1C5D10] (ab233561) at 1/500 dilution + Recombinant human PRKAG3 protein (amino acids 9-151)


    (Expected MW is 41.1 kDa)

References

ab233561 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233561.
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