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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKA
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RecombinantRabMAb

Recombinant Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)

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  • Certificate of Compliance
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Western blot - Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)
  • Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPMAYR1-127] to PRKAR1A (phospho S77) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)

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Overview

  • Product name

    Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free
    See all PRKAR1A primary antibodies
  • Description

    Rabbit monoclonal [EPMAYR1-127] to PRKAR1A (phospho S77) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects Protein Kinase A regulatory subunit I alpha/PRKAR1A phosphorylated at serine 77.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PRKAR1A (phospho S77). The exact sequence is proprietary.

  • General notes

    ab248879 is the carrier-free version of ab139682 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248879 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Ozgur Ogut at the Mayo Clinic.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPMAYR1-127
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKA
    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cAMP
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Associated products

  • Alternative Versions

    • Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] (ab139682)
    • Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

Applications

Our Abpromise guarantee covers the use of ab248879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Tissue specificity

      Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
    • Involvement in disease

      Defects in PRKAR1A are the cause of Carney complex type 1 (CNC1) [MIM:160980]. CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.
      Defects in PRKAR1A are the cause of intracardiac myxoma (INTMYX) [MIM:255960]. Inheritance is autosomal recessive.
      Defects in PRKAR1A are the cause of primary pigmented nodular adrenocortical disease type 1 (PPNAD1) [MIM:610489]. Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. PPNAD1 is most often diagnosed in patients with Carney complex, but it can also be observed in patients without other manifestations or familial history.
    • Sequence similarities

      Belongs to the cAMP-dependent kinase regulatory chain family.
      Contains 2 cyclic nucleotide-binding domains.
    • Post-translational
      modifications

      The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
    • Target information above from: UniProt accession P10644 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5573 Human
      • Entrez Gene: 19084 Mouse
      • Entrez Gene: 25725 Rat
      • Omim: 188830 Human
      • SwissProt: P10644 Human
      • SwissProt: Q9DBC7 Mouse
      • SwissProt: P09456 Rat
      • Unigene: 280342 Human
      • Unigene: 30039 Mouse
      • Unigene: 10990 Rat
      see all
    • Alternative names

      • ACRDYS1 antibody
      • ADOHR antibody
      • cAMP dependent protein kinase regulatory subunit alpha 1 antibody
      • cAMP dependent protein kinase regulatory subunit RIalpha antibody
      • cAMP dependent protein kinase type I alpha regulatory chain antibody
      • cAMP dependent protein kinase type I alpha regulatory subunit antibody
      • cAMP-dependent protein kinase type I-alpha regulatory subunit antibody
      • CAR antibody
      • Carney complex type 1 antibody
      • CNC 1 antibody
      • CNC antibody
      • CNC1 antibody
      • DKFZp779L0468 antibody
      • KAP0_HUMAN antibody
      • MGC17251 antibody
      • PKA RIA antibody
      • PKR 1 antibody
      • PKR1 antibody
      • PPNAD 1 antibody
      • PPNAD1 antibody
      • PRKAR 1 antibody
      • PRKAR1 antibody
      • PRKAR1A antibody
      • PRKAR1A/RARA FUSION GENE antibody
      • Protein kinase A type 1a regulatory subunit antibody
      • Protein kinase cAMP dependent regulatory type I alpha antibody
      • PTC2 CHIMERIC ONCOGENE, INCLUDED antibody
      • Tissue specific extinguisher 1 antibody
      • Tissue-specific extinguisher 1 antibody
      • TSE 1 antibody
      • TSE1 antibody
      see all

    Images

    • Western blot - Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)
      Western blot - Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)
      All lanes : Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] (ab139682) at 1/1000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : Fetal heart lysate treated with Alkaline Phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 43 kDa



      This data was developed using ab139682, the same antibody clone in a different buffer formulation.

       

    • Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)
      Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab248879? Please let us know so that we can cite the reference in this datasheet.

    ab248879 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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