Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit Monoclonal [EPMAYR1-111] to PRKAR1A (phospho S83 + S83) - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
Product nameAnti-PRKAR1A (phospho S83 + S83) antibody [EPMAYR1-111] - BSA and Azide free
See all PRKAR1A primary antibodies
DescriptionRabbit Monoclonal [EPMAYR1-111] to PRKAR1A (phospho S83 + S83) - BSA and Azide free
This antibody only detects Protein Kinase A regulatory subunit I alpha/PRKAR1A phosphorylated at serine 83.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human PRKAR1A (phospho S83). The exact sequence is proprietary.
Database link: P10644
ab249153 is the carrier-free version of ab154851 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab249153 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Ozgur Ogut at the Mayo Clinic. Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab249153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.|
Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Involvement in diseaseDefects in PRKAR1A are the cause of Carney complex type 1 (CNC1) [MIM:160980]. CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.
Defects in PRKAR1A are the cause of intracardiac myxoma (INTMYX) [MIM:255960]. Inheritance is autosomal recessive.
Defects in PRKAR1A are the cause of primary pigmented nodular adrenocortical disease type 1 (PPNAD1) [MIM:610489]. Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. PPNAD1 is most often diagnosed in patients with Carney complex, but it can also be observed in patients without other manifestations or familial history.
Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
modificationsThe pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
- Information by UniProt
- ACRDYS1 antibody
- ADOHR antibody
- cAMP dependent protein kinase regulatory subunit alpha 1 antibody
All lanes : Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851) at 1/1000 dilution
Lane 1 : Rat heart lysate; untreated
Lane 2 : Rat heart lysate; treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
This data was developed using ab154851, the same antibody clone in a different buffer formulation.
Lower panel shows labeling with a non-phospho Protein Kinase A regulatory subunit I alpha/PRKAR1A antibody.
This data was developed using ab154851, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Protein Kinase A regulatory subunit I alpha/PRKA1A with ab154851 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using ab154851, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Protein Kinase A regulatory subunit I alpha/PRKAR1A with ab154851 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using ab154851, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154851 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab249153 has not yet been referenced specifically in any publications.