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RecombinantRabMAb

Recombinant Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)

Submit a review Submit a question References (3)
Western blot - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
  • Western blot - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
  • Western blot - Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
  • Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8491] to PRKAR1A+PRKAR1B
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-PRKAR1A+PRKAR1B antibody [EPR8491]
    See all PRKAR1A+PRKAR1B primary antibodies

  • Description

    Rabbit monoclonal [EPR8491] to PRKAR1A+PRKAR1B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PRKAR1A+PRKAR1B aa 200-300. The exact sequence is proprietary. Within PRKAR1A + PRKAR1B.

  • Positive control

    • WB: Recombinant Human PRKAR1A and PRKAR1B protein; Human, mouse, rat brain lysate, U-87 MG whole cell lysate; IHC-P: Mouse liver, human liver carcinoma, and rat kidney tissue sections.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P
1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody is recommended.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P
1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody is recommended.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Images

  • Western blot - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    Western blot - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    All lanes : Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695) at 1/10000 dilution (Purified)

    Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa



    The doublets should be PRKAR1A isoforms.

  • Western blot - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    Western blot - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695) at 1/10000 dilution (Purified) + Human brain lysate

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 43 kDa



    The doublets should be PRKAR1A isoforms.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling PRKAR1A+PRKAR1B with Purified ab139695 at 1/300 dilution (1.29 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling PRKAR1A+PRKAR1B with Purified ab139695 at 1/300 dilution (1.29 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling PRKAR1A+PRKAR1B with Purified ab139695 at 1/300 dilution (1.29 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    Western blot - Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    All lanes : Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695) at 1/10000 dilution

    Lane 1 : Recombinant human full length PRKAR1A protein
    Lane 2 : Recombinant human full length PRKAR1B protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa



    Buffer and concentration: 5% NFDM/TBST

    Exposure time

    Lane 1: 10 seconds

    Lande 2: 40 seconds

    Observed band

    Lane 1: 51KDa

    Lane 2: 52 KDa

     

  • Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
    Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)

References (3)

Publishing research using ab139695? Please let us know so that we can cite the reference in this datasheet.

ab139695 has been referenced in 3 publications.

  • Liu Y  et al. PRKAR1A deficiency impedes hypertrophy and reduces heart size. Physiol Rep 8:e14405 (2020). PubMed: 32212257
  • He J  et al. Recurrent somatic mutations ofPRKAR1Ain isolated cardiac myxoma. Oncotarget 8:103968-103974 (2017). PubMed: 29262613
  • Wang S  et al. PRKAR1A is a functional tumor suppressor inhibiting ERK/Snail/E-cadherin pathway in lung adenocarcinoma. Sci Rep 6:39630 (2016). IHC-P ; Human . PubMed: 27995993

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