Recombinant Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8491] to PRKAR1A+PRKAR1B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PRKAR1A+PRKAR1B antibody [EPR8491]
See all PRKAR1A+PRKAR1B primary antibodies -
Description
Rabbit monoclonal [EPR8491] to PRKAR1A+PRKAR1B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PRKAR1A+PRKAR1B aa 200-300. The exact sequence is proprietary. Within PRKAR1A + PRKAR1B.
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Positive control
- WB: Recombinant Human PRKAR1A and PRKAR1B protein; Human, mouse, rat brain lysate, U-87 MG whole cell lysate; IHC-P: Mouse liver, human liver carcinoma, and rat kidney tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8491 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
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IHC-P |
1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa. |
IHC-P
1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended. |
Images
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All lanes : Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695) at 1/10000 dilution (Purified)
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDaThe doublets should be PRKAR1A isoforms.
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Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695) at 1/10000 dilution (Purified) + Human brain lysate
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 43 kDaThe doublets should be PRKAR1A isoforms.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling PRKAR1A+PRKAR1B with Purified ab139695 at 1/300 dilution (1.29 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling PRKAR1A+PRKAR1B with Purified ab139695 at 1/300 dilution (1.29 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling PRKAR1A+PRKAR1B with Purified ab139695 at 1/300 dilution (1.29 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Western blot - Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695)All lanes : Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695) at 1/10000 dilution
Lane 1 : Recombinant human full length PRKAR1A protein
Lane 2 : Recombinant human full length PRKAR1B protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDaBuffer and concentration: 5% NFDM/TBST
Exposure time
Lane 1: 10 seconds
Lande 2: 40 seconds
Observed band
Lane 1: 51KDa
Lane 2: 52 KDa
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab139695 has been referenced in 3 publications.
- Liu Y et al. PRKAR1A deficiency impedes hypertrophy and reduces heart size. Physiol Rep 8:e14405 (2020). PubMed: 32212257
- He J et al. Recurrent somatic mutations ofPRKAR1Ain isolated cardiac myxoma. Oncotarget 8:103968-103974 (2017). PubMed: 29262613
- Wang S et al. PRKAR1A is a functional tumor suppressor inhibiting ERK/Snail/E-cadherin pathway in lung adenocarcinoma. Sci Rep 6:39630 (2016). IHC-P ; Human . PubMed: 27995993