Key features and details
- Mouse polyclonal to PRKAR1B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRKAR1B antibody
DescriptionMouse polyclonal to PRKAR1B
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human PRKAR1B aa 1-381.
MASPPACPSEEDESLKGCELYVQLHGIQQVLKDCIVHLCISKPERPMKFL REHFEKLEKEENRQILARQKSNSQSDSHDEEVSPTPPNPVVKARRRRGGV SAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFAHLDDNERSDIF DAMFPVTHIAGETVIQQGNEGDNFYVVDQGEVDVYVNGEWVTNISEGGSF GELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFL SKVSILESLEKWERLTVADALEPVQFEDGEKIVVQGEPGDDFYIITEGTA SVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLKCVKL DRPRFERVLGPCSEILKRNIQRYNSFISLTV
Database link: NP_002726.1
- PRKAR1B transfected 293T cell lysate; HeLa cells
This product was previously labelled as PKA regulatory subunit I beta
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab167325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
modificationsThe pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
- Information by UniProt
- cAMP dependent protein kinase type I beta regulatory subunit antibody
- cAMP-dependent protein kinase type I-beta regulatory subunit antibody
- KAP1_HUMAN antibody
All lanes : Anti-PRKAR1B antibody (ab167325) at 1 µg/ml
Lane 1 : PRKAR1B transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : Goat Anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 43 kDa
Immunofluorescent analysis of PRKAR1B expression in HeLa cells using ab167325 at 10 µg/ml dilution.
ab167325 has not yet been referenced specifically in any publications.