Product nameAnti-PRKAR2 antibody
DescriptionRabbit polyclonal to PRKAR2
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PRKAR2 aa 50-100. The exact sequence is proprietary.
Database link: P13861
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab264220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 2 - 5 µg/ml.|
|IHC-P||1/200 - 1/1000.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
|WB||1/2000 - 1/10000.|
RelevancecAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of AMPK. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
- cAMP dependent protein kinase regulatory subunit RII alpha antibody
- PKR2 antibody
- PRKAR antibody
PRKAR2 was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264220 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab264220 at 1 µg/ml.
Lane 1: ab264220 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
All lanes : Anti-PRKAR2 antibody (ab264220) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PRKAR2 using ab264220 at 1/200 dilution in immunohistochemical analysis. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264220 has not yet been referenced specifically in any publications.