Product nameAnti-PRKAR2B antibody [EP2648]
See all PRKAR2B primary antibodies
DescriptionRabbit monoclonal [EP2648] to PRKAR2B
Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PRKAR2B aa 1-100 (N terminal). The exact sequence is proprietary.
- Fetal brain, human testis and NIH/3T3 cell lysates. Human brain tissue.
This product was previously labelled as PKA 2 beta (regulatory subunit)
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22649251|
|WB||1/5000 - 1/20000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
|Flow Cyt||1/20 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRegulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
modificationsPhosphorylated by the activated catalytic chain.
Cellular localizationCytoplasm. Cell membrane. Co-localizes with PJA2 in the cytoplasm and at the cell membrane.
- Information by UniProt
- AI451071 antibody
- AW061005 antibody
- cAMP dependent protein kinase type II beta regulatory chain antibody
All lanes : Anti-PRKAR2B antibody [EP2648] (ab75996) at 1/20000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Human testis lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Overlay histogram showing SHSY-5Y cells stained with ab75996 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75996, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab75996, at 1/100 dilution, staining PRKAR2B in paraffin-embedded human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Yin C et al. PID1 alters the antilipolytic action of insulin and increases lipolysis via inhibition of AKT/PKA pathway activation. PLoS One 14:e0214606 (2019). Read more (PubMed: 30990811) »
- Anastácio A et al. Protein profile of mouse ovarian follicles grown in vitro. Mol Hum Reprod 23:827-841 (2017). Read more (PubMed: 29069483) »