Key features and details
- Rabbit polyclonal to PRKRIR
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PRKRIR antibody
See all PRKRIR primary antibodies
DescriptionRabbit polyclonal to PRKRIR
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide (Human) corresponding to a region between residues 150 and 200 of human PRKRIR (NP_004696).
- Whole cell lysate from HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).|
|IP||Use a concentration of 1 - 4 µg/ml.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevancePRKRIR is upstream regulator of interferon induced serine/threonine protein kinase R (PKR). It may block the PKR inhibitory function of P58IPK, resulting in the restoration of kinase activity and suppression of cell growth.
- 52 kDa repressor of the inhibitor of the protein kinase antibody
- 58 kDa interferon induced protein kinase interacting protein antibody
- DAP4 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling PRKRIR with ab70334 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-PRKRIR antibody (ab70334) at 0.04 µg/ml
Lane 1 : whole cell lysate of HeLa cells at 50 µg
Lane 2 : whole cell lysate of HeLa cells at 15 µg
Lane 3 : whole cell lysate of HeLa cells at 5 µg
Lane 4 : whole cell lysate of NIH3T3 cells at 50 µg
Predicted band size: 88 kDa
Observed band size: 88 kDa
IP/WB - 1 mg whole cell lysate of HeLa cells used, and 1/4 of the reaction loaded.
Lane 1: ab70333 used at 3µg/ml lysate for IP.
Lane 2: ab70334 used at 3µg/ml lysate for IP.
Lane 3: IgG control.
ab70334 used at at 1µg/ml for the subsequent WB for all lanes.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab70334 has not yet been referenced specifically in any publications.