Anti-PRKX antibody (ab154739)
Key features and details
- Rabbit polyclonal to PRKX
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-PRKX antibody
See all PRKX primary antibodies -
Description
Rabbit polyclonal to PRKX -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to a region within amino acids 44-309 of Human PRKX (Uniprot ID: P51817).
-
Positive control
- 293T, A431 and Mouse brain whole cell lysates, H3347 xenograft tissue
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8. |
Target
-
Tissue specificity
High levels in adult and fetal brain, kidney and lung; low levels in adult placenta, heart, liver, skeletal muscle, pancreas and fetal liver. -
Involvement in disease
Note=A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. - Information by UniProt
-
Database links
- Entrez Gene: 5613 Human
- Entrez Gene: 19108 Mouse
- Entrez Gene: 501563 Rat
- Omim: 300083 Human
- SwissProt: P51817 Human
- SwissProt: Q922R0 Mouse
- Unigene: 390788 Human
- Unigene: 290338 Mouse
-
Alternative names
- PKX1 antibody
- PRKX antibody
- PRKX_HUMAN antibody
see all
Images
-
Anti-PRKX antibody (ab154739) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 41 kDa
10% SDS PAGE -
Anti-PRKX antibody (ab154739) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 41 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human H3347 xenograft tissue labeling PRKX with ab154739 at 1/500 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab154739 has not yet been referenced specifically in any publications.