Overview

  • Product name

    Anti-PRKX antibody [EPR4840]
    See all PRKX primary antibodies
  • Description

    Rabbit monoclonal [EPR4840] to PRKX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PRKX aa 300-400. The exact sequence is proprietary.

  • Positive control

    • FC: permeabilized A549 cells; WB: A549, Jurkat, 293T, fetal kidney and fetal brain lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4840
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Tissue specificity

      High levels in adult and fetal brain, kidney and lung; low levels in adult placenta, heart, liver, skeletal muscle, pancreas and fetal liver.
    • Involvement in disease

      Note=A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links

    • Alternative names

      • PKX1 antibody
      • PRKX antibody
      • PRKX_HUMAN antibody
      • Protein kinase PKX1 antibody
      • Serine/threonine-protein kinase PRKX antibody
      see all

    Images

    • All lanes : Anti-PRKX antibody [EPR4840] (ab109389) at 1/1000 dilution

      Lane 1 : A549 cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : 293T cell lysates
      Lane 4 : Human fetal kidney lysates
      Lane 5 : Human fetal brain lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 41 kDa

    References

    This product has been referenced in:

    • Tang B  et al. PEA15 promotes liver metastasis of colorectal cancer by upregulating the ERK/MAPK signaling pathway. Oncol Rep 41:43-56 (2019). Read more (PubMed: 30365128) »
    • Ouyang C  et al. Transforming Growth Factor (TGF)-ß-activated Kinase 1 (TAK1) Activation Requires Phosphorylation of Serine 412 by Protein Kinase A Catalytic Subunit a (PKACa) and X-linked Protein Kinase (PRKX). J Biol Chem 289:24226-37 (2014). WB . Read more (PubMed: 25028512) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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