Key features and details
- Rabbit polyclonal to PRL-1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRL-1 antibody
See all PRL-1 primary antibodies
DescriptionRabbit polyclonal to PRL-1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human PRL-1 aa 23-156.
- Human placenta tissue; Transfected HEK293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
FunctionProtein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis.
Tissue specificityExpressed in bone marrow, lymph nodes, T lymphocytes, spleen, thymus and tonsil. Overexpressed in tumor cell lines.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain.
Developmental stageExpressed in fetal liver.
modificationsFarnesylated. Farnesylation is required for membrane targeting. Unfarnesylated forms are shifted into the nucleus.
Cellular localizationCell membrane. Early endosome. Endoplasmic reticulum. Cytoplasm. Cytoplasm > cytoskeleton > spindle. And mitotic spindle.
- Information by UniProt
- HH 72 antibody
- HH72 antibody
- Hypothetical protein DKFZp779M0721 antibody
ab121185, at 1/75 dilution, staining PRL-1 in paraffin-embedded Human placenta tissue, by Immunohistochemistry.
All lanes : Anti-PRL-1 antibody (ab121185) at 1/250 dilution
Lane 1 : Negative control (vector-only transfected HEK293T lysate)
Lane 2 : PRL-1-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
ab121185 has not yet been referenced specifically in any publications.