Product nameAnti-PRMT1 antibody
See all PRMT1 primary antibodies
DescriptionRabbit polyclonal to PRMT1
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IHC-Fr, IPmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human
Predicted to work with: Cow, Non human primates
- Recombinant mouse PRMT1 protein (ab78937) can be used as a positive control in WB. This antibody gave a positive signal in WB against Caco 2 Whole Cell, SW480 Whole Cell and Mouse Thymus Tissue. In IF against HeLa Cells and IHC against Human Hippocampus
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab73246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42, 39 kDa (predicted molecular weight: 42 kDa).|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24663083|
|IP||Use at an assay dependent concentration.|
FunctionArginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
Cellular localizationNucleus. Cytoplasm > cytosol.
- Information by UniProt
- ANM 1 antibody
- ANM1 antibody
- ANM1_HUMAN antibody
All lanes : Anti-PRMT1 antibody (ab73246) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab76828)
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab76999)
Lane 3 : Thymus (Mouse) Tissue Lysate (ab76823)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa (possible non-specific binding), 38 kDa (possible isoform), 90 kDa (possible non-specific binding)
Exposure time: 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% milk before being incubated with ab73246 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2,3 and 4 of human PRMT1. The predicted molecular weights of isoforms 1,2,3 and 4 are 41kDa, 39kDa, 39kDa and 40kDa respectively.
PRMT1 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to PRMT1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73246.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 42kDa: PRMT1; non specific - 30kDa: We are unsure as to the identity of this extra band.
ICC/IF image of ab73246 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73246, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 1µg/ml.
IHC image of PRMT1 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73246, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
This product has been referenced in:
- Vadnais C et al. GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1. Nat Commun 9:1418 (2018). Read more (PubMed: 29651020) »
- Hsu JH et al. PRMT1-Mediated Translation Regulation Is a Crucial Vulnerability of Cancer. Cancer Res 77:4613-4625 (2017). Read more (PubMed: 28655788) »