Anti-PRMT1 antibody [PRMT1-171] (ab12189)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-PRMT1 antibody [PRMT1-171]
See all PRMT1 primary antibodies -
Description
Mouse monoclonal [PRMT1-171] to PRMT1 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, ELISA, IP, Double Immunodiffusionmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Monkey -
Immunogen
Monoclonal Anti-PRMT1 is derived from the PRMT1-171 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with full length human PRMT1.
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Positive control
- Human kidney cell line 293T.
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General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
PRMT1-171 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab12189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB | ||
| ELISA | ||
| IP | ||
| Double Immunodiffusion |
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 µg/ml (determined by using a whole cell extract of the human kidney cell line 293T). Predicted molecular weight: 42 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the protein arginine N-methyltransferase family. -
Cellular localization
Nucleus. Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 520388 Cow
- Entrez Gene: 3276 Human
- Entrez Gene: 15469 Mouse
- Entrez Gene: 60421 Rat
- Omim: 602950 Human
- SwissProt: Q99873 Human
- SwissProt: Q9JIF0 Mouse
- SwissProt: Q63009 Rat
see all -
Alternative names
- ANM 1 antibody
- ANM1 antibody
- ANM1_HUMAN antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [PRMT1-171] (ab12189)](https://www.abcam.com/ps/products/12/ab12189/Images/ab12189-220993-anti-prmt1-antibody-prmt1-171-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [PRMT1-171] (ab12189)Image from Butler JS et al., J Biol Chem. 2011;286(14):12234-44. Fig S6.; doi: 10.1074/jbc.M110.202416.Immunocytochemsitry/Immunofluorescence analysis of MEFs labelling PRMT1 with ab12189. +/+ and PRMT1−/flox MEFs were treated with 2 μg/ml 4-hydroxytamoxifen (OHT) or untreated for 3 days. The cells were then seeded onto glass coverslips and cultured in fresh medium lacking OHT for 3 additional days. The cells were rinsed with ice cold methanol, fixed with 2% paraformaldehyde, and permeabilized with methanol -20°C for 5 minutes. The cells were first blocked for 20 minutes at room temperature (0.5% gelatin, 1× PBS) and then incubated with primary antibodies followed by washes (0.1% gelatin, 1% normal goat serum, 1X PBS) and incubation with Alexa Fluor 594-conjugated rabbit secondary antibody.
Protocols
References
This product has been referenced in:
- Vadnais C et al. GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1. Nat Commun 9:1418 (2018). Read more (PubMed: 29651020) »
- Butler JS et al. Protein-arginine Methyltransferase 1 (PRMT1) Methylates Ash2L, a Shared Component of Mammalian Histone H3K4 Methyltransferase Complexes. J Biol Chem 286:12234-44 (2011). ICC/IF ; Human . Read more (PubMed: 21285357) »
