Key features and details
- Rabbit polyclonal to PRMT2/HMT1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRMT2/HMT1 antibody
DescriptionRabbit polyclonal to PRMT2/HMT1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human PRMT2/HMT1 aa 8-336.
Database link: P55345
- 293T, A431, H1299, HeLaS3, HepG2, Molt4 and Raji whole cell lysates; A431 cells; A549 xenograft
This product was previously labelled as PRMT2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 49 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionArginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
Tissue specificityWidely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.
Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
Contains 1 SH3 domain.
Cellular localizationCytoplasm. Nucleus. Translocates from the cytoplasm to the nucleus, after hormone exposure.
- Information by UniProt
- ANM2_HUMAN antibody
- EC 2.1.1. antibody
- histone arginine N methyltransferase PRMT2 antibody
Anti-PRMT2/HMT1 antibody (ab154154) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 49 kDa
10% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PRMT2/HMT1 with ab154154 at 1/200 dilution. The image in the lower panel was co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling PRMT2/HMT1 with ab154154 at 1/100 dilution.
ab154154 has not yet been referenced specifically in any publications.