Overview

  • Product name

  • Description

    Goat polyclonal to PRMT3
  • Host species

    Goat
  • Specificity

    ab223243 is expected to recognize all reported isoforms (NP_005779.1; NP_001138638.1; NP_001138639.1).
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PRMT3 aa 408-421 (internal sequence). (NP_005779.1).
    Sequence:

    DPKTLISEPCGIKH


    Database link: O60678

  • Positive control

    • WB: MCF7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab223243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).

Target

  • Function

    Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family.
    Contains 1 C2H2-type zinc finger.
  • Domain

    The zinc-finger is responsible for substrate specificity.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2010005E20Rik antibody
    • 2410018A17Rik antibody
    • AL033309 antibody
    • ANM3_HUMAN antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 antibody
    • HMT1 hnRNP methyltransferase like 3 antibody
    • HMT1 hnRNP methyltransferase like 3 S. cerevisiae antibody
    • HMT1-like 3 antibody
    • hnRNP methyltransferase like 3 antibody
    • HRMT1L3 antibody
    • PRMT 3 antibody
    • PRMT3 antibody
    • Protein arginine methyltransferase 3 antibody
    • Protein arginine N methyltransferase 3 antibody
    • Protein arginine N-methyltransferase 3 antibody
    see all

Images

  • Anti-PRMT3 antibody (ab223243) at 1 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223243 has not yet been referenced specifically in any publications.

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