Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMethylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
Contains 1 C2H2-type zinc finger.
DomainThe zinc-finger is responsible for substrate specificity.
- Information by UniProt
- 2010005E20Rik antibody
- 2410018A17Rik antibody
- AL033309 antibody
All lanes : Anti-PRMT3 antibody (ab91430) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 30 seconds
SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91430.
20% of the immunoprecipitate was loaded per lane, and probed with ab91430 at 1µg/ml (lane 1) or a control IgG (lane2).
Detection: chemoluminescence with an exposure time of 30 seconds.
This product has been referenced in:
- Mizuno K & Sloboda RD Protein arginine methyltransferases interact with intraflagellar transport particles and change location during flagellar growth and resorption. Mol Biol Cell 28:1208-1222 (2017). Read more (PubMed: 28298486) »