Product nameAnti-PRMT3 antibody [EPR13279]
See all PRMT3 primary antibodies
DescriptionRabbit monoclonal [EPR13279] to PRMT3
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PRMT3 aa 1-100. The exact sequence is proprietary.
Database link: O60678
- WB: HeLa, HepG2, 293 and MCF7 cell lysates IHC: Human cervix carcinoma tissue ICC/IF: MCF7 cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab191562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).|
|IHC-P||1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionMethylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
Contains 1 C2H2-type zinc finger.
DomainThe zinc-finger is responsible for substrate specificity.
- Information by UniProt
- 2010005E20Rik antibody
- 2410018A17Rik antibody
- AL033309 antibody
All lanes : Anti-PRMT3 antibody [EPR13279] (ab191562) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded, human cervix carcinoma tissue labeling PRMT3 with ab191562 at a 1/100 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling PRMT3 with ab191562 at a 1/50 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor® 488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-PRMT3 at a 1/50 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.
Western blot analysis on immunoprecipitation from 1) 293 cell lysate and 2) PBS, labeling PRMT3 using ab191562 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
ab191562 has not yet been referenced specifically in any publications.