RecombinantRabMAb

Recombinant Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

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Immunocytochemistry/ Immunofluorescence - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
  • Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
  • Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13279] to PRMT3 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PRMT3 antibody [EPR13279] - BSA and Azide free
    See all PRMT3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13279] to PRMT3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide within Human PRMT3 aa 1-100. The exact sequence is proprietary.
    Database link: O60678

    Synthetic peptide within Human PRMT3 aa 1-100. The exact sequence is proprietary.
    Database link: O60678

  • General notes

    Ab240322 is the carrier-free version of ab191562. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240322 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab240322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.

  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family.
    Contains 1 C2H2-type zinc finger.

  • Domain

    The zinc-finger is responsible for substrate specificity.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • 2010005E20Rik antibody
      • 2410018A17Rik antibody
      • AL033309 antibody
      • ANM3_HUMAN antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 3 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase like 3 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 3 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 antibody
      • HMT1 hnRNP methyltransferase like 3 antibody
      • HMT1 hnRNP methyltransferase like 3 S. cerevisiae antibody
      • HMT1-like 3 antibody
      • hnRNP methyltransferase like 3 antibody
      • HRMT1L3 antibody
      • PRMT 3 antibody
      • PRMT3 antibody
      • Protein arginine methyltransferase 3 antibody
      • Protein arginine N methyltransferase 3 antibody
      • Protein arginine N-methyltransferase 3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
      Immunocytochemistry/ Immunofluorescence - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

      Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling PRMT3 with ab191562 at a 1/50 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor® 488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-PRMT3 at a 1/50 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191562).

    • Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
      Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

      Western blot analysis on immunoprecipitation from 1) 293 cell lysate and 2) PBS, labeling PRMT3 using ab191562 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191562).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

      Immunohistochemical analysis of paraffin-embedded, human cervix carcinoma tissue labeling PRMT3 with ab191562 at a 1/100 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191562).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
      Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240322? Please let us know so that we can cite the reference in this datasheet.

    ab240322 has not yet been referenced specifically in any publications.

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