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    prmt3-antibody-epr13279-bsa-and-azide-free-ab240322.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
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RecombinantRabMAb

Recombinant Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

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Immunocytochemistry/ Immunofluorescence - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
  • Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
  • Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13279] to PRMT3 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

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Recombinant human PRMT3 protein (ab167953)
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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-PRMT3 antibody [EPR13279] - BSA and Azide free
    See all PRMT3 primary antibodies
  • Description

    Rabbit monoclonal [EPR13279] to PRMT3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240322 is the carrier-free version of ab191562.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13279
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Arginine Methylation

Associated products

  • Alternative Versions

    • Anti-PRMT3 antibody [EPR13279] (ab191562)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant human PRMT3 protein (ab167953)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family.
    Contains 1 C2H2-type zinc finger.
  • Domain

    The zinc-finger is responsible for substrate specificity.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession O60678 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10196 Human
    • Omim: 603190 Human
    • SwissProt: O60678 Human
    • Unigene: 152337 Human
    • Alternative names

      • 2010005E20Rik antibody
      • 2410018A17Rik antibody
      • AL033309 antibody
      • ANM3_HUMAN antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 3 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase like 3 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 3 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 antibody
      • HMT1 hnRNP methyltransferase like 3 antibody
      • HMT1 hnRNP methyltransferase like 3 S. cerevisiae antibody
      • HMT1-like 3 antibody
      • hnRNP methyltransferase like 3 antibody
      • HRMT1L3 antibody
      • PRMT 3 antibody
      • PRMT3 antibody
      • Protein arginine methyltransferase 3 antibody
      • Protein arginine N methyltransferase 3 antibody
      • Protein arginine N-methyltransferase 3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
      Immunocytochemistry/ Immunofluorescence - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

      Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling PRMT3 with ab191562 at a 1/50 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor® 488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-PRMT3 at a 1/50 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191562).

    • Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
      Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

      Western blot analysis on immunoprecipitation from 1) 293 cell lysate and 2) PBS, labeling PRMT3 using ab191562 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191562).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

      Immunohistochemical analysis of paraffin-embedded, human cervix carcinoma tissue labeling PRMT3 with ab191562 at a 1/100 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191562).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)
      Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab240322? Please let us know so that we can cite the reference in this datasheet.

    ab240322 has not yet been referenced specifically in any publications.

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