Overview

  • Product name

  • Description

    Rabbit polyclonal to PRMT5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 297-534 of Human PRMT5.

  • Positive control

    • 293T, A431, Jurkat and Raji cell lysates; Mahlavu xenograft; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab151321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family.
  • Post-translational
    modifications

    Disulfide bonds and non-covalent association mediate homooligomers formation.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 72 kDa ICln binding protein antibody
    • 72 kDa ICln-binding protein antibody
    • ANM5_HUMAN antibody
    • Histone synthetic lethal 7, S. cerevisiae, homolog of antibody
    • Histone-arginine N-methyltransferase PRMT5 antibody
    • HMT1 hnRNP methyltransferase like 5 antibody
    • HOMOLOG OF; SKB1 antibody
    • HRMT1L5 antibody
    • IBP72 antibody
    • Jak-binding protein 1 antibody
    • JBP 1 antibody
    • JBP1 antibody
    • PRMT 5 antibody
    • PRMT5 antibody
    • Protein arginine methyltransferase 5 antibody
    • Protein arginine N methyltransferase 5 antibody
    • Protein arginine N methyltransferase 5 N terminally processed antibody
    • Protein arginine N-methyltransferase 5 antibody
    • S. POMBE antibody
    • S. POMBE HOMOLOG OF; SKB1 antibody
    • SHK1 KINASE BINDING PROTEIN 1 antibody
    • Shk1 kinase binding protein 1 homolog antibody
    • Shk1 kinase-binding protein 1 homolog antibody
    • Shk1 kinase/binding protein 1, S. pombe, homolog of antibody
    • SKB 1 antibody
    • SKB1 antibody
    • SKB1 homolog antibody
    • SKB1: SKB1 homolog (S. pombe) antibody
    • SKB1Hs antibody
    see all

Images

  • Anti-PRMT5 antibody (ab151321) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 73 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PRMT5 with ab151321 at 1/200 dilution. Lower image co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human Mahlavu xenograft labeling PRMT5 with ab151321 at 1/100 dilution.

References

This product has been referenced in:

  • Qi H  et al. Sirtuin 7-mediated deacetylation of WD repeat domain 77 (WDR77) suppresses cancer cell growth by reducing WDR77/PRMT5 transmethylase complex activity. J Biol Chem 293:17769-17779 (2018). Read more (PubMed: 30282801) »
See 1 Publication for this product

Customer reviews and Q&As

Western blot

Excellent
Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 3-8% Tris-Acetate Gel 1.0 mm)
Sample
Human Cell lysate - whole cell (U2OS)
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Melanie Blasius

Verified customer

Submitted May 20 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up