Anti-PRMT5 antibody (ab151321)
Key features and details
- Rabbit polyclonal to PRMT5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PRMT5 antibody
See all PRMT5 primary antibodies -
Description
Rabbit polyclonal to PRMT5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 297-534 of Human PRMT5.
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Positive control
- 293T, A431, Jurkat and Raji cell lysates; Mahlavu xenograft; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 73 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol
using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 73 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the protein arginine N-methyltransferase family. -
Post-translational
modificationsDisulfide bonds and non-covalent association mediate homooligomers formation. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 515594 Cow
- Entrez Gene: 10419 Human
- Entrez Gene: 27374 Mouse
- Entrez Gene: 100294711 Pig
- Entrez Gene: 364382 Rat
- Omim: 604045 Human
- SwissProt: A7YW45 Cow
- SwissProt: O14744 Human
see all -
Alternative names
- 72 kDa ICln binding protein antibody
- 72 kDa ICln-binding protein antibody
- ANM5_HUMAN antibody
see all
Images
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Anti-PRMT5 antibody (ab151321) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 73 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PRMT5 with ab151321 at 1/200 dilution. Lower image co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human Mahlavu xenograft labeling PRMT5 with ab151321 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151321 has been referenced in 1 publication.
- Qi H et al. Sirtuin 7-mediated deacetylation of WD repeat domain 77 (WDR77) suppresses cancer cell growth by reducing WDR77/PRMT5 transmethylase complex activity. J Biol Chem 293:17769-17779 (2018). PubMed: 30282801