Product nameAnti-PRMT5 antibody - C-terminal
See all PRMT5 primary antibodies
DescriptionRabbit polyclonal to PRMT5 - C-terminal
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Xenopus laevis, Zebrafish
- WB: HL60, HEK-293 and NIH/3T3 whole cell lysate. IHC-P: Human prostate tissue. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab31751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 19584108|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).Can be blocked with Human PRMT5 peptide (ab30553).
ab31751 shows weak staining in mouse samples.
|IHC-P||1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionArginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
modificationsDisulfide bonds and non-covalent association mediate homooligomers formation.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 72 kDa ICln binding protein antibody
- 72 kDa ICln-binding protein antibody
- ANM5_HUMAN antibody
All lanes : Anti-PRMT5 antibody - C-terminal (ab31751) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney cell line) whole cell lysate
Lane 3 :
NIH 3T3 whole cell lysate (ab7179)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 6 minutes
Image courtesy of Human Protein Atlas
ab31751 staining PRMT5 in human prostate, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human skin tissue was incubated with ab31751 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6..
ab31751 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for PRMT5 (red) using ab31751 (0.5 µg/ml) in ICC/IF.
This product has been referenced in:
- Webb LM et al. PRMT5-Selective Inhibitors Suppress Inflammatory T Cell Responses and Experimental Autoimmune Encephalomyelitis. J Immunol 198:1439-1451 (2017). WB ; Mouse . Read more (PubMed: 28087667) »
- Mizuno K & Sloboda RD Protein arginine methyltransferases interact with intraflagellar transport particles and change location during flagellar growth and resorption. Mol Biol Cell 28:1208-1222 (2017). Read more (PubMed: 28298486) »