Overview

  • Product name

    Anti-PRMT5 antibody [EPR5772] - BSA and Azide free
    See all PRMT5 primary antibodies
  • Description

    Rabbit monoclonal [EPR5772] to PRMT5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PRMT5 aa 50-150.

  • Positive control

    • WB: HEK293, HepG2, HeLa, and NIH3T3 cell lysates; mouse and rat brain tissue lysate. ICC/IF: HepG2 and HeLa cells. IHC-P: human infiltrating duct carcinoma of breast tissue, mouse liver tissue. Flow Cyt: HeLa cells.
  • General notes

    Ab215364 is the carrier-free version of ab109451. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab215364 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 72 kDa (predicted molecular weight: 73 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family.
  • Post-translational
    modifications

    Disulfide bonds and non-covalent association mediate homooligomers formation.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 72 kDa ICln binding protein antibody
    • 72 kDa ICln-binding protein antibody
    • ANM5_HUMAN antibody
    • Histone synthetic lethal 7, S. cerevisiae, homolog of antibody
    • Histone-arginine N-methyltransferase PRMT5 antibody
    • HMT1 hnRNP methyltransferase like 5 antibody
    • HOMOLOG OF; SKB1 antibody
    • HRMT1L5 antibody
    • IBP72 antibody
    • Jak-binding protein 1 antibody
    • JBP 1 antibody
    • JBP1 antibody
    • PRMT 5 antibody
    • PRMT5 antibody
    • Protein arginine methyltransferase 5 antibody
    • Protein arginine N methyltransferase 5 antibody
    • Protein arginine N methyltransferase 5 N terminally processed antibody
    • Protein arginine N-methyltransferase 5 antibody
    • S. POMBE antibody
    • S. POMBE HOMOLOG OF; SKB1 antibody
    • SHK1 KINASE BINDING PROTEIN 1 antibody
    • Shk1 kinase binding protein 1 homolog antibody
    • Shk1 kinase-binding protein 1 homolog antibody
    • Shk1 kinase/binding protein 1, S. pombe, homolog of antibody
    • SKB 1 antibody
    • SKB1 antibody
    • SKB1 homolog antibody
    • SKB1: SKB1 homolog (S. pombe) antibody
    • SKB1Hs antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PRMT5 (green) with purified ab109451 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/50) and secondary antibody Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/400).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109451).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labelling PRMT5 with purified ab109451 at 1/100. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109451).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human infiltrating duct carcinoma of breast tissue sections labelling PRMT5 with purified ab109451 at 1/100. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109451).

  • ICC/IF image of ab109451 (unpurified) stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109451, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109451).

  • Overlay histogram showing HeLa cells stained with ab109451 (unpurified, red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109451, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109451).

  • Equilibrium disassociation constant (KD)

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109451).

References

This product has been referenced in:

  • Goyal A  et al. Generation of human induced pluripotent stem cells using epigenetic regulators reveals a germ cell-like identity in partially reprogrammed colonies. PLoS One 8:e82838 (2013). ICC/IF ; Human . Read more (PubMed: 24349377) »
  • Gurung B  et al. Menin directly represses gli1 expression independent of canonical hedgehog signaling. Mol Cancer Res 11:1215-22 (2013). Read more (PubMed: 23928057) »
See all 2 Publications for this product

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