Overview

  • Product name

    Anti-PRMT5 antibody - N-terminal
    See all PRMT5 primary antibodies
  • Description

    Rabbit polyclonal to PRMT5 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human PRMT5 (N terminal). The exact sequence is proprietary.
    Database link: O14744

  • Positive control

    • IHC-P: Mouse intestine and kidney tissues; MCF7 xenograft tissue. ICC/IF: A431 cells. WB: Raji whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.

Target

  • Function

    Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family.
  • Post-translational
    modifications

    Disulfide bonds and non-covalent association mediate homooligomers formation.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 72 kDa ICln binding protein antibody
    • 72 kDa ICln-binding protein antibody
    • ANM5_HUMAN antibody
    • Histone synthetic lethal 7, S. cerevisiae, homolog of antibody
    • Histone-arginine N-methyltransferase PRMT5 antibody
    • HMT1 hnRNP methyltransferase like 5 antibody
    • HOMOLOG OF; SKB1 antibody
    • HRMT1L5 antibody
    • IBP72 antibody
    • Jak-binding protein 1 antibody
    • JBP 1 antibody
    • JBP1 antibody
    • PRMT 5 antibody
    • PRMT5 antibody
    • Protein arginine methyltransferase 5 antibody
    • Protein arginine N methyltransferase 5 antibody
    • Protein arginine N methyltransferase 5 N terminally processed antibody
    • Protein arginine N-methyltransferase 5 antibody
    • S. POMBE antibody
    • S. POMBE HOMOLOG OF; SKB1 antibody
    • SHK1 KINASE BINDING PROTEIN 1 antibody
    • Shk1 kinase binding protein 1 homolog antibody
    • Shk1 kinase-binding protein 1 homolog antibody
    • Shk1 kinase/binding protein 1, S. pombe, homolog of antibody
    • SKB 1 antibody
    • SKB1 antibody
    • SKB1 homolog antibody
    • SKB1: SKB1 homolog (S. pombe) antibody
    • SKB1Hs antibody
    see all

Images

  • Anti-PRMT5 antibody - N-terminal (ab227457) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 73 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded mouse intestine tissue stained for PRMT5 using ab227457 at 1/500 dilution in immunohistochemical analysis.

  • A431 (human epithelial cell line from cervix adenocarcinoma) cells stained for PRMT5 (green) using ab227457 at 1/500 dilution in ICC/IF. Cells were fixed in paraformaldehyde.

    Lower panel costained with Hoechst 33342.

     

  • Paraffin-embedded MCF7 (human) xenograft tissue stained for PRMT5 using ab227457 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse kidney tissue stained for PRMT5 using ab227457 at 1/500 dilution in immunohistochemical analysis.

References

ab227457 has not yet been referenced specifically in any publications.

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