Overview

  • Product name
    Anti-PRMT6 antibody [2C3-F1-G1]
    See all PRMT6 primary antibodies
  • Description
    Mouse monoclonal [2C3-F1-G1] to PRMT6
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PRMT6. expressed in E.coli
    Database link: NP_060607.2

  • Positive control
    • HeLa and 293T cell lysates; HeLa cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab151191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 41 kDa.
ICC/IF 1/300.

Target

  • Function
    Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53. Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).
  • Tissue specificity
    Highly expressed in kidney and testis.
  • Sequence similarities
    Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT6 subfamily.
    Contains 1 SAM-dependent MTase PRMT-type domain.
  • Post-translational
    modifications
    Automethylation enhances its stability and antiretroviral activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANM6_HUMAN antibody
    • Chromobox protein homolog 7 antibody
    • FLJ10559 antibody
    • FLJ51477 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6 antibody
    • Histone-arginine N-methyltransferase PRMT6 antibody
    • HMT1 hnRNP methyltransferase like 6. antibody
    • HRMT1L6 antibody
    • OTTHUMP00000012633 antibody
    • PRMT 6 antibody
    • prmt6 antibody
    • Protein arginine methyltransferase 6 antibody
    • Protein arginine N methyltransferase 6 antibody
    • Protein arginine N-methyltransferase 6 antibody
    see all

Images

  • All lanes : Anti-PRMT6 antibody [2C3-F1-G1] (ab151191) at 1/1000 dilution

    Lane 1 : COS7 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : 3T3 cell lysate
    Lane 5 : HeLa cell lysate

    Predicted band size: 41 kDa

  • Immunohistochemical staining of paraffin-embedded human breast cancer using anti-PRMT6 (ab151191)  at a dilution of 1:200. This image was produced with antibody produced in ascites. 

  • Immunofluorescent analysis of HeLa cells labeling PRMT6 with ab151191 at 1/300 dilution. This image was produced with antibody produced in ascites. 

  • All lanes : Anti-PRMT6 antibody [2C3-F1-G1] (ab151191) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate

    Predicted band size: 41 kDa



    This image was produced with antibody produced in ascites. 

References

ab151191 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Immortalized mouse striatal cells (STHdh Q7/Q7))
Gel Running Conditions
Reduced Denaturing (10% polyacrylamide gel)
Loading amount
40 µg
Specification
Immortalized mouse striatal cells (STHdh Q7/Q7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 07 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up