Overview

  • Product name

  • Description

    Rabbit polyclonal to PRMT7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 408-665 of Human PRMT7 (Q9NVM4).

  • Positive control

    • PC3 whole cell lysate; Rat RT2, Hela and Mouse C2C12 xenograft tissues.
  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab126965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 78 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.
  • Involvement in disease

    Defects in PRMT7 are associated with mild intellectual disabilityy, obesity and symmetrical shortening of the digits and posterior metacarpals and metatarsals. The phenotype is a phenocopy of pseudohypoparathyroidism (PHP).
  • Sequence similarities

    Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.
    Contains 2 SAM-dependent MTase PRMT-type domains.
  • Cellular localization

    Cytoplasm, cytosol. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • [Myelin basic protein]-arginine N-methyltransferase PRMT7 antibody
    • ANM7_HUMAN antibody
    • FLJ10640 antibody
    • Histone-arginine N-methyltransferase PRMT7 antibody
    • KIAA1933 antibody
    • Myelin basic protein arginine N methyltransferase antibody
    • OTTHUMP00000174863 antibody
    • PRMT7 antibody
    • Protein arginine methyltransferase 7 antibody
    • Protein arginine N-methyltransferase 7 antibody
    see all

Images

  • Anti-PRMT7 antibody (ab126965) at 1/2000 dilution + PC3 whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 78 kDa



    7.5% SDS PAGE
  • ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Rat RT2 xenograft tissue by Immunohistochemistry.
  • ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Human Hela xenograft tissue by Immunohistochemistry.
  • ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Mouse C2C12 xenograft tissue by Immunohistochemistry.

References

This product has been referenced in:

See 1 Publication for this product

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