Overview

  • Product name

    Anti-PRMT7 antibody [EPR13495]
    See all PRMT7 primary antibodies
  • Description

    Rabbit monoclonal [EPR13495] to PRMT7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PRMT7 aa 550-650. The exact sequence is proprietary.
    Database link: Q9NVM4

  • Positive control

    • HepG2, HeLa, 293 and MCF7 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
ICC/IF 1/100 - 1/250.

Target

  • Function

    Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.
  • Involvement in disease

    Defects in PRMT7 are associated with mild intellectual disabilityy, obesity and symmetrical shortening of the digits and posterior metacarpals and metatarsals. The phenotype is a phenocopy of pseudohypoparathyroidism (PHP).
  • Sequence similarities

    Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.
    Contains 2 SAM-dependent MTase PRMT-type domains.
  • Cellular localization

    Cytoplasm, cytosol. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • [Myelin basic protein]-arginine N-methyltransferase PRMT7 antibody
    • ANM7_HUMAN antibody
    • FLJ10640 antibody
    • Histone-arginine N-methyltransferase PRMT7 antibody
    • KIAA1933 antibody
    • Myelin basic protein arginine N methyltransferase antibody
    • OTTHUMP00000174863 antibody
    • PRMT7 antibody
    • Protein arginine methyltransferase 7 antibody
    • Protein arginine N-methyltransferase 7 antibody
    see all

Images

  • All lanes : Anti-PRMT7 antibody [EPR13495] (ab181214) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 78 kDa

  • Immunofluorescent analysis of HeLa cells (-20℃ acetone-fixed) labeling PRMT7 with ab181214 at 1/250 dilution (red). Goat anti rabbit IgG (Alexa Fluor® 555) was used as a secondary antibody, at a dilution of 1/200. Counter stain: Dapi (blue).

References

ab181214 has not yet been referenced specifically in any publications.

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