Anti-PRMT8 antibody (ab168134)
Key features and details
- Mouse polyclonal to PRMT8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRMT8 antibody -
Description
Mouse polyclonal to PRMT8 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Full length protein, corresponding to amino acids 1-394 of Human PRMT8 (NP_062828.3).
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Positive control
- PRMT8 transfected 293T cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab168134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. |
Target
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Function
Membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS. -
Tissue specificity
Brain-specific. -
Sequence similarities
Belongs to the protein arginine N-methyltransferase family. PRMT8 subfamily. -
Domain
The SH3-binding motifs mediate the interaction with SH3 domain-containing proteins such as PRMT2 and FYN, possibly leading to displace the N-terminal domain and activate the protein.
The N-terminal region (1-60) inhibits the enzymatic activity. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 56341 Human
- Entrez Gene: 381813 Mouse
- Omim: 610086 Human
- SwissProt: Q9NR22 Human
- SwissProt: Q6PAK3 Mouse
- Unigene: 504530 Human
- Unigene: 39750 Mouse
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Alternative names
- ANM8_HUMAN antibody
- Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 4 antibody
- Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4 antibody
see all
Images
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Western blot - Anti-PRMT8 antibody (ab168134)All lanes : Anti-PRMT8 antibody (ab168134) at 1 µg/ml
Lane 1 : PRMT8 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 45 kDa
Datasheets and documents
References (1)
ab168134 has been referenced in 1 publication.
- Kagoya Y et al. Arginine methylation of FOXP3 is crucial for the suppressive function of regulatory T cells. J Autoimmun 97:10-21 (2019). PubMed: 30318155
