Anti-PRMT8 antibody (ab168134)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-PRMT8 antibody
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DescriptionMouse polyclonal to PRMT8
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Host speciesMouse
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Full length protein, corresponding to amino acids 1-394 of Human PRMT8 (NP_062828.3).
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Positive control
- PRMT8 transfected 293T cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.20
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. |
Target
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FunctionMembrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.
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Tissue specificityBrain-specific.
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Sequence similaritiesBelongs to the protein arginine N-methyltransferase family. PRMT8 subfamily.
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DomainThe SH3-binding motifs mediate the interaction with SH3 domain-containing proteins such as PRMT2 and FYN, possibly leading to displace the N-terminal domain and activate the protein.
The N-terminal region (1-60) inhibits the enzymatic activity. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 56341 Human
- Entrez Gene: 381813 Mouse
- Omim: 610086 Human
- SwissProt: Q9NR22 Human
- SwissProt: Q6PAK3 Mouse
- Unigene: 504530 Human
- Unigene: 39750 Mouse
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Alternative names
- ANM8_HUMAN antibody
- Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 4 antibody
- Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4 antibody
see all
Images
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Western blot - Anti-PRMT8 antibody (ab168134)All lanes : Anti-PRMT8 antibody (ab168134) at 1 µg/ml
Lane 1 : PRMT8 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 45 kDa
Protocols
Datasheets and documents
References
ab168134 has not yet been referenced specifically in any publications.
