Anti-PRMT8 antibody (ab168134)

Submit a review Submit a question References (1)

Overview

  • Product name

    Anti-PRMT8 antibody

  • Description

    Mouse polyclonal to PRMT8

  • Host species

    Mouse

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Full length protein, corresponding to amino acids 1-394 of Human PRMT8 (NP_062828.3).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • PRMT8 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab168134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.

Target

  • Function

    Membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.

  • Tissue specificity

    Brain-specific.

  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family. PRMT8 subfamily.

  • Domain

    The SH3-binding motifs mediate the interaction with SH3 domain-containing proteins such as PRMT2 and FYN, possibly leading to displace the N-terminal domain and activate the protein.
    The N-terminal region (1-60) inhibits the enzymatic activity.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ANM8_HUMAN antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 4 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4 antibody
      • HMT1 hnRNP methyltransferase like 3 antibody
      • HMT1 hnRNP methyltransferase like 4 antibody
      • HRMT1 L3 antibody
      • HRMT1 L4 antibody
      • HRMT1L 3 antibody
      • HRMT1L 4 antibody
      • HRMT1L3 antibody
      • HRMT1L4 antibody
      • prmt8 antibody
      • Protein arginine N methyltransferase 4 antibody
      • Protein arginine N methyltransferase 8 antibody
      • Protein arginine N-methyltransferase 8 antibody
      see all

    Images

    • Western blot - Anti-PRMT8 antibody (ab168134)
      Western blot - Anti-PRMT8 antibody (ab168134)
      All lanes : Anti-PRMT8 antibody (ab168134) at 1 µg/ml

      Lane 1 : PRMT8 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 15 µl per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size: 45 kDa

    References

    This product has been referenced in:

    • Kagoya Y  et al. Arginine methylation of FOXP3 is crucial for the suppressive function of regulatory T cells. J Autoimmun 97:10-21 (2019). Read more (PubMed: 30318155) »
    See 1 Publication for this product

    Publishing research using ab168134? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab168134.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com