Anti-pro Caspase-12 antibody (ab8118)
Key features and details
- Rabbit polyclonal to pro Caspase-12
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-pro Caspase-12 antibody
See all pro Caspase-12 primary antibodies -
Description
Rabbit polyclonal to pro Caspase-12 -
Host species
Rabbit -
Specificity
The antibody only recognizes the prodomain, not the activated form. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Mouse pro Caspase-12 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O08736 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa.
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa. |
Target
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Relevance
Caspase 12 is activated by ER stress, including disruption of ER calcium homeostasis and mediates ER stress-induced apoptosis. Caspase 12 is co-localized to the ER with several proteins that are involved in Alzheimers disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase 12 mediates cytotoxicity induced by amyloid-beta. It is ubiquitously expressed in mouse tissue. -
Cellular localization
Endoplasmic reticulum -
Database links
- Entrez Gene: 120329 Human
- Entrez Gene: 12364 Mouse
- Omim: 608633 Human
- SwissProt: Q6UXS9 Human
- SwissProt: O08736 Mouse
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Alternative names
- CASP12 antibody
- CASP12P1 antibody
Images
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab8118 has been referenced in 5 publications.
- Liu N et al. TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease. J Neuroinflammation 19:26 (2022). PubMed: 35093118
- Tang Y et al. Activation of PPARγ Protects Obese Mice from Acute Lung Injury by Inhibiting Endoplasmic Reticulum Stress and Promoting Mitochondrial Biogenesis. PPAR Res 2022:7888937 (2022). PubMed: 36213491
- Jiang J et al. The phosphoinositide 3-kinase inhibitor ZSTK474 increases the susceptibility of osteosarcoma cells to oncolytic vesicular stomatitis virus VSVΔ51 via aggravating endoplasmic reticulum stress. Bioengineered 12:11847-11857 (2021). PubMed: 34720036
- Feng SQ et al. VEGF Antagonism Attenuates Cerebral Ischemia/Reperfusion-Induced Injury via Inhibiting Endoplasmic Reticulum Stress-Mediated Apoptosis. Biol Pharm Bull 42:692-702 (2019). PubMed: 30828041
- Hauri-Hohl MM et al. Donor T-cell alloreactivity against host thymic epithelium limits T-cell development after bone marrow transplantation. Blood 109:4080-8 (2007). PubMed: 17213290