Product namePro-Apoptosis Bcl Family Antibody Sampler Panel
ab228527, Apoptosis Antibody Sampler Panel, is a collection of 1 anti-rabbit (HRP) secondary antibody and 6 recombinant rabbit monoclonal antibodies against Bad, Bax, Bcl-2, Bcl-XL, MCL1, and PUMA provided in 10 µl trial sizes.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Carrier-free formulations are also available for easy conjugation to labels of your choice. Please refer to the ‘Associated products’ section below.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit ab32445 - Anti-Bad antibody [Y208] 1 x 10µl ab32503 - Anti-Bax antibody [E63] 1 x 10µl ab182858 - Anti-Bcl-2 antibody [EPR17509] 1 x 10µl ab32370 - Anti-Bcl-XL antibody [E18] 1 x 10µl ab32087 - Anti-MCL1 antibody [Y37] 1 x 10µl ab33906 - Anti-PUMA antibody [EP512Y] 1 x 10µl ab205718 - Goat Anti-Rabbit IgG H+L (HRP) 1 x 100µg
- Anti-MCL1 antibody [Y37] - BSA and Azide free (ab186822)
- Anti-PUMA antibody [EP512Y] - BSA and Azide free (ab186917)
- Anti-MCL1 antibody [Y37] (Alexa Fluor® 647) (ab197035)
- Anti-MCL1 antibody [Y37] (Alexa Fluor® 488) (ab197529)
- Anti-Bcl-XL antibody [E18] - BSA and Azide free (ab199099)
- Anti-Bad antibody [Y208] (Biotin) (ab199754)
- Anti-Bcl-XL antibody [E18] (PE) (ab208747)
- Anti-MCL1 antibody [Y37] (PE) (ab209289)
- Anti-PUMA antibody [EP512Y] (PE) (ab211689)
- Anti-PUMA antibody [EP512Y] (Alexa Fluor® 488) (ab214237)
- Anti-Bax antibody [E63] - BSA and Azide free (ab216985)
- Anti-Bcl-2 antibody [EPR17509] - BSA and Azide free (ab219608)
- Anti-Bad antibody [Y208] - BSA and Azide free (ab220116)
- Anti-CD28 antibody [E18] (PE) (ab64638)
Immunocytochemistry/Immunofluorescence analysis of H1299 cells labelling MCL1 with unpurified ab32087. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% Serum for 1 hour at 24°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 24°C. The secondary antibody was an Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/2000. DAPI (blue) was used as the nuclear counterstain.
ab33906 at a 1:250 dilution staining PUMA in Hela cells using Immunocytochemistry/Immunofluorescence.
Cytoplasm, nuclear membrane and nucleus staining on lymphocytes and cancer cells of Human endometrial cancer tissue is observed.
Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody followed by ab97051 at 1/500.
Immunohistochemistry of human primary melanoma, staining Bcl-XL (red) with unpurified ab32370.
Antigen retrieval was performed in EDTA/Tris buffer (pH 8) before being blocked with 10%NGS for one hour at room temperature. Samples were incubated with primary antibody (1/50) at room temperature for one hour. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody.
IHC image of ab32503 staining Bax in rat kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32503, 1:250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemical analysis of 2% paraformaldehyde-fixed,0.1% Triton-X100 permeabilized RAW 264.7 labeling Bad with ab32445 at 1/250dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) secondary antibody at 1/1000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228527 has not yet been referenced specifically in any publications.