Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16883] to pro Caspase-1 + p10 + p12 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free
See all pro Caspase-1 + p10 + p12 primary antibodies
DescriptionRabbit monoclonal [EPR16883] to pro Caspase-1 + p10 + p12 - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human pro Caspase-1 + p10 + p12 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P29466
- WB: Rat lung and spleen lysate. Human fetal lung lysate. J774A.1 and RAW 264.7 whole cell lysate.
Ab238972 is the carrier-free version of ab179515. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab238972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab238972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 45, 42, 35, 12, 10 kDa (predicted molecular weight: 45, 42, 35, 12, 10 kDa).|
|IP||Use at an assay dependent concentration.|
- CASP1 antibody
- Caspase 1 apoptosis related cysteine peptidase antibody
- ICE antibody
pro Caspase1 + p10 + p12 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab179515 at 1/100 dilution.
Western blot was performed from the immunoprecipitate using ab179515 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).
Lane 2: ab179515 IP in RAW 264.7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179515).
All lanes : Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free (ab238972) at 1/1000 dilution
Lane 1 : Rat lung lysate
Lane 2 : Human fetal lung lysate
Lane 3 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
Lane 4 : Rat spleen lysate
Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 45, 42, 35, 12, 10 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 30 seconds; Lane 2,3 and 4: 10 seconds; Lane 5: 8 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab179515).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238972 has not yet been referenced specifically in any publications.