Product nameAnti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free
See all pro Caspase-1 + p10 + p12 primary antibodies
DescriptionRabbit monoclonal [EPR16883] to pro Caspase-1 + p10 + p12 - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human pro Caspase-1 + p10 + p12 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P29466
- WB: Rat lung and spleen lysate. Human fetal lung lysate. J774A.1 and RAW 264.7 whole cell lysate.
Ab238972 is the carrier-free version of ab179515. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab238972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab238972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 45, 42, 35, 12, 10 kDa (predicted molecular weight: 45, 42, 35, 12, 10 kDa).|
|IP||Use at an assay dependent concentration.|
- CASP1 antibody
- Caspase 1 apoptosis related cysteine peptidase antibody
- ICE antibody
pro Caspase1 + p10 + p12 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab179515 at 1/100 dilution.
Western blot was performed from the immunoprecipitate using ab179515 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).
Lane 2: ab179515 IP in RAW 264.7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179515).
All lanes : Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free (ab238972) at 1/1000 dilution
Lane 1 : Rat lung lysate
Lane 2 : Human fetal lung lysate
Lane 3 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
Lane 4 : Rat spleen lysate
Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 45, 42, 35, 12, 10 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 30 seconds; Lane 2,3 and 4: 10 seconds; Lane 5: 8 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab179515).
ab238972 has not yet been referenced specifically in any publications.