Recombinant
RabMAb

Recombinant Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free (ab238972)

Overview

  • Product name

    Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free
    See all pro Caspase-1 + p10 + p12 primary antibodies
  • Description

    Rabbit monoclonal [EPR16883] to pro Caspase-1 + p10 + p12 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human pro Caspase-1 + p10 + p12 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P29466

  • Positive control

    • WB: Rat lung and spleen lysate. Human fetal lung lysate. J774A.1 and RAW 264.7 whole cell lysate.
  • General notes

    Ab238972 is the carrier-free version of ab179515. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16883
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab238972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 45, 42, 35, 12, 10 kDa (predicted molecular weight: 45, 42, 35, 12, 10 kDa).
IP Use at an assay dependent concentration.

Target

Images

  • pro Caspase1 + p10 + p12 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab179515 at 1/100 dilution.

    Western blot was performed from the immunoprecipitate using ab179515 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).

    Lane 2: ab179515 IP in RAW 264.7 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of  ab179515 in RAW 264.7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179515).

  • All lanes : Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free (ab238972) at 1/1000 dilution

    Lane 1 : Rat lung lysate
    Lane 2 : Human fetal lung lysate
    Lane 3 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45, 42, 35, 12, 10 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 30 seconds; Lane 2,3 and 4: 10 seconds; Lane 5: 8 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab179515).

References

ab238972 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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