Anti-pro Caspase-12 antibody (ab8117)
Key features and details
- Rabbit polyclonal to pro Caspase-12
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-pro Caspase-12 antibody
See all pro Caspase-12 primary antibodies -
Description
Rabbit polyclonal to pro Caspase-12 -
Host species
Rabbit -
Specificity
The antibody only recognizes the prodomain, not the activated form. -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 2 µg/ml.
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa.
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Notes |
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IHC-P
Use a concentration of 2 µg/ml. |
ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa. |
Target
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Relevance
Caspase 12 is activated by ER stress, including disruption of ER calcium homeostasis and mediates ER stress-induced apoptosis. Caspase 12 is co-localized to the ER with several proteins that are involved in Alzheimers disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase 12 mediates cytotoxicity induced by amyloid-beta. It is ubiquitously expressed in mouse tissue. -
Cellular localization
Endoplasmic reticulum -
Database links
- Entrez Gene: 12364 Mouse
- SwissProt: O08736 Mouse
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Alternative names
- CASP12 antibody
- CASP12P1 antibody
Images
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All lanes : Anti-pro Caspase-12 antibody (ab8117) at 1 µg/ml
Lane 1 : Murine brain tissue lysate with absence of blocking peptide
Lane 2 : Murine brain tissue lysate with presence of blocking peptide -
ab8117 staining Caspase-12 in mouse liver tissue by IHC
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Immunofluorescence of Caspase-12 in Mouse Liver cells using ab8117 at 10 ug/ml.
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab8117 has been referenced in 6 publications.
- Wang S et al. LncRNA H19 inhibits ER stress induced apoptosis and improves diabetic cardiomyopathy by regulating PI3K/AKT/mTOR axis. Aging (Albany NY) 14:6809-6828 (2022). PubMed: 36044268
- Ji J et al. Activating PPARβ/δ Protects against Endoplasmic Reticulum Stress-Induced Astrocytic Apoptosis via UCP2-Dependent Mitophagy in Depressive Model. Int J Mol Sci 23:N/A (2022). PubMed: 36142731
- Hu HJ et al. Hydrogen Sulfide Ameliorates Angiotensin II-Induced Atrial Fibrosis Progression to Atrial Fibrillation Through Inhibition of the Warburg Effect and Endoplasmic Reticulum Stress. Front Pharmacol 12:690371 (2021). PubMed: 34950023
- Liu H et al. YAP promotes gastric cancer cell survival and migration/invasion via the ERK/endoplasmic reticulum stress pathway. Oncol Lett 18:6752-6758 (2019). PubMed: 31807184
- Shen B et al. Mechanism of HN-3 cell apoptosis induced by carboplatin: Combination of mitochondrial pathway associated with Ca2+ and the nucleus pathways. Mol Med Rep 18:4978-4986 (2018). PubMed: 30272304
- Fatma N et al. Deficiency of Prdx6 in lens epithelial cells induces ER stress response-mediated impaired homeostasis and apoptosis. Am J Physiol Cell Physiol 301:C954-67 (2011). PubMed: 21677259