Key features and details
- Rabbit polyclonal to pro Caspase-7
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-pro Caspase-7 antibody
See all pro Caspase-7 primary antibodies
DescriptionRabbit polyclonal to pro Caspase-7
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Rat pro Caspase-7 aa 1-250. (Expressed in E.coli).
MTDDQDCAAELEMADSSTEDGVDAKPDRSTIISSLLWKKKKNASMCPVST TRDRVPTYLYRMDFEKMGKCIIINNKNFDKATGMDVRNGTDKDAEALFKC FRSLGFEVTVYNDCSCAKMQDLLRRASEEDHSNSACFACVLLSHGEENLI YGKDGVTPIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQADSG PINDTDANPRYKIPVEADFLFAYSTVPGXXSWRNPGKGSWFVQALCSILN
Database link: P55210
- IHC-P: Rat stomach tissue. WB: Recombinant rat pro Caspase 7 protein; Rat liver lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231532 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 34 kDa.|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death.
Tissue specificityHighly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Sequence similaritiesBelongs to the peptidase C14A family.
modificationsCleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
- Information by UniProt
- apoptosis-related cysteine peptidase antibody
- Apoptotic protease Mch-3 antibody
- CASP-7 antibody
Anti-pro Caspase-7 antibody (ab231532) at 3 µg/ml + Rat liver lysate
Predicted band size: 34 kDa
Anti-pro Caspase-7 antibody (ab231532) at 2 µg/ml + Recombinant rat pro Caspase 7 protein
Predicted band size: 34 kDa
Paraffin-embedded rat stomach tissue stained for pro caspase 7 using ab231532 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded rat stomach tissue stained for pro caspase 7 using ab231532 at 20 µg/mL in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231532 has not yet been referenced specifically in any publications.